In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites.

Within the current examine, the in vitro metabolic habits of a benzopyridooxathiazepine (BZN), a potent tubulin polymerization inhibitor, was investigated by liquid chromatography-UV detection (LC-UV). First, easy and quick LC-UV strategies have been optimized and validated to judge the pharmacokinetic profile of BZN utilizing rat liver microsomes or hepatocytes main cultures suspensions. Regardless of the medium investigated, baseline decision between the interior commonplace and BZN was achieved in a run time lower than 15min utilizing a Symmetry ODS column (150mm×4.6mm i.d., 5μm) and a cell part consisting of acetonitrile/water/formic acid 60:40:0.1 (v/v/v). Linearity was assessed within the 0.1-50μM and within the 0.05-5μM focus ranges, respectively, in microsomal and hepatocyte matrix.

Based on the novel technique based mostly on the construct of the accuracy profile, whole error of the developed strategies was included inside the ±10% limits of acceptance. Then, from incubation of BZN with each liver microsomes and or hepatocytes, structural informations on part I and part II metabolites have been acquired utilizing liquid chromatography coupled to electrospray orbitrap mass spectrometer (LC-MS). Mass spectrum, double bond equal and elemental composition have been helpful knowledge to entry to the chemical construction of every metabolite.

In microsomal suspension, 4 predominant metabolites have been noticed together with monohydroxylation and dihydroxylation of the benzopyridooxathiazepine core, demethylation of the methoxyphenyl moiety, in addition to their mixtures. The part II metabolites detected in hepatocytes suspension have been the glucuronide adducts of each demethylated BZN and mono-oxygenated BZN. Primarily based on the structural elucidation of the metabolites detected, we proposed an in vitro metabolic pathway of BZN, a brand new tubulin polymerization inhibitor.

In vitro metabolism of BIIB021, an inhibitor of warmth shock protein 90, in liver microsomes and hepatocytes of rats, canines, and people and recombinant human cytochrome P450 isoforms.

Inhibition of warmth shock protein 90 (HSP90) ends in the degradation of oncoproteins that drive malignant development and induce cell loss of life, thus making HSP90 a possible goal of most cancers remedy. 6-Chloro-9-(4-methoxy-3, 5-dimethyl-pyridin-2-ylmethyl)-9H-purin-2-ylamine (BIIB021), an artificial HSP90 inhibitor, exhibited promising antitumor exercise in preclinical fashions. It’s at present in part II medical trials for the oral remedy of breast most cancers. The target of this examine was to acquire each quantitative and qualitative metabolic profiles of [(14)C]BIIB021 in rat, canine, and human liver microsomes and hepatocytes to supply assist for in vivo security and medical research.

The metabolites of [(14)C]BIIB021 have been recognized utilizing liquid chromatography-tandem mass spectrometry coupled with radiometric detection. BIIB021 was extensively metabolized in each liver microsomes and hepatocytes. The foremost oxidative metabolic pathways recognized for all species have been attributable to hydroxylation (M7) and O-demethylation (M2) of the methoxy-dimethylpyridine moiety. The vast majority of M7 in canine hepatocytes was additional conjugated to type the glucuronide (M4). Oxidative dechlorination (M6), monooxygenation (M10), and oxidative N-dealkylation of the methoxy-dimethylpyridine moiety (M11 and M12) have been noticed because the minor metabolic pathways in hepatocytes of all three species. A glutathione conjugate (M18) was additionally recognized in all species.

Its formation was catalyzed, partially, by soluble glutathione transferase by way of direct displacement of the chlorine on the amino-chloropurine moiety. Subsequent minor secondary metabolites M13, M14, M15, and M17 have been noticed in human, canine, and rat hepatocytes. Outcomes from incubations of BIIB021 with human recombinant cytochrome P450 (P450) isoforms and a P450 antibody inhibition examine in human liver microsomes urged that the formation of M7 is especially catalyzed by CYP2C19 and CYP3A4, whereas the formation of minor metabolite M2 in human liver microsomes most likely may very well be attributed to CYP3A4.

In vitro metabolism of rivoglitazone, a novel peroxisome proliferator-activated receptor γ agonist, in rat, monkey, and human liver microsomes and freshly remoted hepatocytes.

The in vitro metabolism of rivoglitazone, (RS)-5-{4-[(6-methoxy-1-methyl-1H-benzimidazol-2-yl)methoxy]benzyl}-1,3-thiazolidine-2,4-dione monohydrochloride, a novel thiazolidinedione (TZD) peroxisome proliferator-activated receptor γ selective agonist, was studied in liver microsomes and freshly remoted hepatocytes of rat, monkey, and human in addition to cDNA-expressed human cytochrome P450 (P450) and UDP-glucuronosyltransferase (UGT) enzymes. Fourteen metabolites have been detected, and these constructions have been elucidated by liquid chromatography-tandem mass spectrometry.

5 preliminary metabolic pathways of rivoglitazone consisting of 4 oxidation pathways and one N-glucuronidation pathway have been predicted in correspondence with these proposed for in vivo research utilizing rats and monkeys. In metabolization utilizing liver microsomes, the TZD ring-opened mercapto amide (M22) and TZD ring-opened mercapto carboxylic acid (M23) have been recognized as the first metabolite of the TZD ring-opening pathway and its sequential metabolite, which haven’t been detected beforehand from in vivo research. Mixture with S-adenosyl-L-methionine was helpful to acquire the sequential S-methylated metabolites from the oxidative metabolites.

Kinesis Adaptor Body 1/2-20 Female x 1/4-28 Female
CHR5105	Scientific Laboratory Supplies	EACH	EUR 55.2

Kinesis Adapter Luer Female to M6 Female; Black PEEK
CHR4317	Scientific Laboratory Supplies	EACH	EUR 22.8

Control Genomic DNA - Rat Female
D1434999-G02	Biochain	100 ug	EUR 170

Female FPLC Connector
FFC-1	ABTBeads	1/pk	EUR 93.6

Female Nut BSPP R/H
GAS1086	Scientific Laboratory Supplies	EACH	EUR 23.14

C-FLEX TUBING, 3'- 1/4" ID, WITH FEMALE MPC CONNECTOR TO FEMALE LUER W/CAP
91-700-04	CORNING	1/pk	EUR 82.8
Description: Flexible Containers; Bioprocess Bags and Parts

Female Nut BSPP L/H 6mm
GAS1092	Scientific Laboratory Supplies	EACH	EUR 15.34

Kinesis Female Luer Fittings
CHR1247	Scientific Laboratory Supplies	PK2	EUR 7.2

Control Genomic DNA - Dog Female
D1734999-G02	Biochain	100 ug	EUR 170

Control Genomic DNA - Human Female
D1234999-G02	Biochain	100 ug	EUR 170

Control Genomic DNA - Mouse Female
D1334999-G02	Biochain	100 ug	EUR 170

Control Genomic DNA - Horse Female
D1O34999-G02	Biochain	100 ug	EUR 170

Kinesis Luer Female - 1/8in Barb
CHR4593	Scientific Laboratory Supplies	EACH	EUR 2.4

Kinesis Female Nut (Micro Fittings)
CHR4137	Scientific Laboratory Supplies	EACH	EUR 12

Control Genomic DNA - Rabbit Female
D1834999-G02	Biochain	100 ug	EUR 170

Control Genomic DNA - Bovine Female
D1B34999-G02	Biochain	100 ug	EUR 170

Kinesis Luer Female - 3/32in Barb
CHR4591	Scientific Laboratory Supplies	EACH	EUR 3.6

Control Genomic DNA - Porcine Female
D1934999-G02	Biochain	100 ug	EUR 170

Control Genomic DNA - Chicken Female
D1C34999-G02	Biochain	100 ug	EUR 170

Control Genomic DNA - Hamster Female
D1H34999-G02	Biochain	100 ug	EUR 170

Kinesis Adapter SS 10.32 Female to 1/4-28 Male
CHR2347	Scientific Laboratory Supplies	EACH	EUR 36

Kinesis 10-32 Female to Male Luer Nut
CHR4311	Scientific Laboratory Supplies	EACH	EUR 16.8

Kinesis Adapter PEEK 10.32 Female to 1/4-28 Male
CHR4333	Scientific Laboratory Supplies	EACH	EUR 38.4

Kinesis Female Luer - M6 Tefzel Black
CHR4363	Scientific Laboratory Supplies	EACH	EUR 8.4

Neurons (iPSC from Blood Cells; Female)
ASE-9321F	Applied StemCell	1 vial (1 x 10^6)	EUR 375.9
Description: 12 month

Age grouped female skin tissue array
SK244a	TissueArray	each	EUR 270
Description: Age grouped female skin tissue array, 12 cases/24 cores, replacing SK244

Kinesis Adaptor Body - 1/4-28 Female x 1/4-28 Male
CHR4339	Scientific Laboratory Supplies	EACH	EUR 51.6

Kinesis Siltite Fingertight Female Nut
CHR1941	Scientific Laboratory Supplies	PK5	EUR 136.8

Kinesis Adapter 1/2-20 Female to 1/4-28 Male; PCTFE
CHR4361	Scientific Laboratory Supplies	EACH	EUR 39.6

Control Genomic DNA - Guinea Pig Female
D1G34999-G02	Biochain	100 ug	EUR 170

Kinesis Adapter Kel-F M6 Female to 1/4-28 Male
CHR4337	Scientific Laboratory Supplies	EACH	EUR 39.6

Kinesis Adaptor 5/16-24 Female To 1/4-28 Male; Kel-F
CHR4299	Scientific Laboratory Supplies	EACH	EUR 44.4

Kinesis Adapter 5/16-24 Female to 1/4-28 Male Kel-F
CHR4355	Scientific Laboratory Supplies	EACH	EUR 33.6

Female reproductive system tissue array
FRS801	TissueArray	each	EUR 306
Description: Female reproductive system tissue array, with stage and grade info, 80 cases/80 cores

Kinesis Female Luer to MicroTight Body
CHR4323	Scientific Laboratory Supplies	EACH	EUR 75.6

V-Neck Polycotton Lab Coat - Female - 100cm/40in - Medium
SAF0078	Scientific Laboratory Supplies	EACH	EUR 61.2

Kinesis Adapter Body PEEK 10.32 Female to 1/4-28 Male
CHR4335	Scientific Laboratory Supplies	EACH	EUR 33.6

Kinesis Female Nut 5/16-24 PEEK Black
CHR4139	Scientific Laboratory Supplies	EACH	EUR 12

Kinesis Female Luer to 10-32 Male PEEK
CHR4419	Scientific Laboratory Supplies	EACH	EUR 46.8

Kinesis Adaptor PTFE 1/4-28 Female x 1/4-28 Male long
CHR4373	Scientific Laboratory Supplies	EACH	EUR 43.2

Kinesis Luer Assembly M6 Female to Male
CHR4313	Scientific Laboratory Supplies	EACH	EUR 24

Kinesis Adaptor 1/4-28 Female to M6 Male
CHR4375	Scientific Laboratory Supplies	EACH	EUR 40.8

Control Genomic DNA - Rhesus Monkey Female
D1534999-G02	Biochain	100 ug	EUR 170

Kinesis Female Luer Fitting System - 1/8
CHR4545	Scientific Laboratory Supplies	EACH	EUR 7.2

Kinesis Female Luer Fitting System - 1/16
CHR4553	Scientific Laboratory Supplies	EACH	EUR 7.2

Adapter X2 M30x1.5 female to NPT 3/4" male
8890035	Scientific Laboratory Supplies	PK2	EUR 169.2

Adapter X2 M30x1.5 female to NPT 3/4" male
8890083	Scientific Laboratory Supplies	PK2	EUR 258

Kinesis Adapter PEEK 10.32 Male to M6 Female
CHR4301	Scientific Laboratory Supplies	EACH	EUR 22.8

Kinesis Adapter PEEK 10.32 Female to M6 Male
CHR4633	Scientific Laboratory Supplies	EACH	EUR 39.6

Kinesis Adapter Male luer to 10-32 Female PEEK
CHR4309	Scientific Laboratory Supplies	EACH	EUR 21.6

Control Genomic DNA - Cynomolgus Monkey Female
D1534999-Cy-G02	Biochain	100 ug	EUR 170

Kinesis Adapter Male luer to M6 Female ETFE
CHR4351	Scientific Laboratory Supplies	EACH	EUR 16.8

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female
CHR4303	Scientific Laboratory Supplies	EACH	EUR 22.8

Kinesis Female Nut PEEK Black 1/4-28 - 1/8
CHR3357	Scientific Laboratory Supplies	EACH	EUR 12

Female reproductive system tumor tissue array
FE951	TissueArray	each	EUR 354
Description: Female reproductive system tumor tissue array, including pathology grade, TNM and clinical stage (AJCC 8.0), 95 cases/95 cores (core size 1.5mm), Recent Year Collection

Kinesis Adapter Male Luer to 1/4-28 Female PEEK
CHR4307	Scientific Laboratory Supplies	EACH	EUR 21.6

Kinesis Adapter Male luer to 1/4-28 Female ETFE
CHR4347	Scientific Laboratory Supplies	EACH	EUR 14.4

Astrocytes Mature (iPSC from Blood Cells; Female)
ASE-9322MF	Applied StemCell	1 vial (1 x 10^6)	EUR 546
Description: 12 month

Normal female dog multiple organs tissue array
DGF281	TissueArray	each	EUR 306
Description: Normal female dog multiple organs tissue array, 28 organs/ 28cores

Neural Stem Cells (iPSC from Blood Cells; Female)
ASE-9234F	Applied StemCell	1 vial (1 x 10^6)	EUR 497.4
Description: 12 month

Kinesis Female Nut PEEK Nat for 1/16in Tubing
CHR4141	Scientific Laboratory Supplies	EACH	EUR 13.2

Kinesis Adapter Female luer to Male 10-32 ETFE
CHR4287	Scientific Laboratory Supplies	EACH	EUR 8.4

Kinesis Adapter Female Luer to Male 1/4-28; PP
CHR4241	Scientific Laboratory Supplies	EACH	EUR 8.4

Kinesis Adapter PEEK 10.32 Flat Male to 1/4-28 Female
CHR4341	Scientific Laboratory Supplies	EACH	EUR 39.6

Neurons Starter Kit (iPSC from Blood Cells; Female)
ASE-9321KF	Applied StemCell	1 Kit	EUR 473.1
Description: 12 month

Tubing 1mMetal insulated 2 fittings M16x1 female
8930221	Scientific Laboratory Supplies	KIT	EUR 340.8

Dopaminergic Neurons (iPSC from Blood Cells; Female)
ASE-9323F	Applied StemCell	1 vial	EUR 448.8
Description: 12 month

Kinesis Adapter Female Luer to Male 1/4-28; ETFE
CHR4255	Scientific Laboratory Supplies	EACH	EUR 8.4

Kinesis Adaptor; Female Luer to 5/16-24 Male; ETFE
CHR4319	Scientific Laboratory Supplies	EACH	EUR 8.4

Kinesis Adapter PP; Barb 1/16 to Luer Lock Female
CHR4589	Scientific Laboratory Supplies	EACH	EUR 2.4

Astrocytes Precursors (iPSC from Blood Cells; Female)
ASE-9322PF	Applied StemCell	1 vial (1 x 10^6)	EUR 448.8
Description: 12 month

Kinesis Adaptor 5/16-24 Male To 1/4-28 Female; Kel-F
CHR4417	Scientific Laboratory Supplies	EACH	EUR 39.6

Kinesis Adapter Female Luer to Male 1/4-28; Delrin
CHR4231	Scientific Laboratory Supplies	EACH	EUR 7.2

V-Neck Polycotton Lab Coat - Female - 92cm/36in - Small
SAF0074	Scientific Laboratory Supplies	EACH	EUR 62.4

V-Neck Polycotton Lab Coat - Female - 96cm/38in - Medium
SAF0076	Scientific Laboratory Supplies	EACH	EUR 60

Multiplex ELISA Kit For Human Female Hormone - (8-plex)
MEK1022	BosterBio	1 kit	EUR 1696.8

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female; Extra Long
CHR4305	Scientific Laboratory Supplies	EACH	EUR 25.2

Kinesis Adapter Assembly 1/8 NPT x 1/4-28 Female PEEK Red
CHR5065	Scientific Laboratory Supplies	EACH	EUR 25.2

Kinesis Adapter Female Luer to Male 1/4-28; PP; Large Bore
CHR4243	Scientific Laboratory Supplies	EACH	EUR 8.4

Kinesis Female Luer to Barb Adapter 1/16ID Tubing; Tefzel
CHR4605	Scientific Laboratory Supplies	EACH	EUR 7.2

Kinesis Adapter Assembly 1/4in NPT x 1/4-28 Female PEEK Red
CHR5053	Scientific Laboratory Supplies	EACH	EUR 30

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female)
ASE-9323KF	Applied StemCell	1 vial	EUR 546
Description: 12 month

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female)
ASE-9322PKF	Applied StemCell	1 Kit	EUR 497.4
Description: 12 month

C-FLEX TUBING, 3'- 1/4" ID, WITH MALE LUER WITH CAP TO FEMALE LUER
91-700-00	CORNING	1/pk	EUR 79.2
Description: Flexible Containers; Bioprocess Bags and Parts

CELLSTACK,FILLING ACCESSORY,3/8 IN C-FLEX,FEMALE MPC,S,1/4
11902	CORNING	1/pk	EUR 337.2
Description: Stackable Cell Culture; Closed Systems Cell Culture Chambers

Monkey Capuchin (Cebus) control (non-immunized) serum, Male and Female
NMCB-1	Alpha Diagnostics	0.5 ml	EUR 196.8

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I
T6734700-G02-1	Biochain	1 slide	EUR 239

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I
T6734700-G02-5	Biochain	5 slides	EUR 883

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I
T6834700-G02-1	Biochain	1 slide	EUR 239

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I
T6834700-G02-5	Biochain	5 slides	EUR 883

Elbow fittings x2 90° M16x1 female/male stainless steel side length 2x54mm
8970442	Scientific Laboratory Supplies	PK2	EUR 262.8

CELLSTACK,FILLING ACC,33MM CAP,MPC FEMALE QK CON,W/DUSTCVR,3/8"ID,S,IND,1/4
3329	CORNING	1/pk	EUR 337.2
Description: Stackable Cell Culture; CellSTACK

A-431 Cell Slide (Human (85 yrs, Female) skin epidermoid carcinoma) (5 slides/pk)
HCLS-17002	Alpha Diagnostics	1 pk	EUR 300

HeLa Cell Slide (Human (31 yrs, Female) cervix epithelioid carcinoma) (5 slides/pk)
HCLS-17001	Alpha Diagnostics	1 pk	EUR 300

Option quick disconnect kit, includes 2 male/female attachments & silicone tubing
V0020-QD	Benchmark Scientific	1 PC	EUR 144.9

Monkey Marmoset (Callithrix jacchus) control (non-immunized) serum, Male and Female
NMMS-1	Alpha Diagnostics	0.5 ml	EUR 270

Option quick disconnect kit, includes 2 male/female attachments and silicone tubing
BCM1550	Bio Basic	1 pcs, 1 UNIT	EUR 244.87

L1210 Cell Slide (DBA subline 212 Mouse (8 months, Female) lymphocytic leukemia) (5 slides/pk)
MCLS-17204	Alpha Diagnostics	1 pk	EUR 300

Monkey African Green (Chlorocebus) control (non-immunized) serum, Male and Female Available
NMAG-1	Alpha Diagnostics	1 ml	EUR 169.2

Corning 33mm Filling Cap 1/4inch (6.4mm) ID Tubing a Male MPC Coupling and a Female MPC End Cap
3333	Scientific Laboratory Supplies	PK4	EUR 355.2

Corning 33mm Filling Cap with a Female MPC with a 1/4inch (6.4mm) ID Coupling and a Male MPC End Cap
3328	Scientific Laboratory Supplies	PK4	EUR 333.6

MCF7 Cell Slide (Human (69 yrs, Female) mammary gland, breast, epithelial adenocarcinoma) (5 slides/pk)
HCLS-17012	Alpha Diagnostics	1 pk	EUR 300

K-562 Cell Slide (Human (53yrs, Female) bone marrow, chronic myelogenous leukemia (CML)) (5 slides/pk)
HCLS-17004	Alpha Diagnostics	1 pk	EUR 300

C-FLEX TUBING, 2'-1/4" ID, WITH FEMALE MPC CONNECTOR WITH 1/4" BARB WITH PINCH CLAMP AND FILLING BELL
91-700-12	CORNING	1/pk	EUR 231.6
Description: Flexible Containers; Bioprocess Bags and Parts

Corning 33mm Filling Cap 1/8inch ID Tubing and a Female Luer 1/8inch Hose Barb a Male Luer Lock Plug
3282	Scientific Laboratory Supplies	PK5	EUR 267.6

HL-60 Cell Slide (Human (36yrs, Female) peripheral blood promyeloblast, acute promyelocytic leukemia) (5 slides/pk)
HCLS-17009	Alpha Diagnostics	1 pk	EUR 300

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from 4 different donors, 2 male and 2 female. 1 ug/ul concentration
R1234148-10-P04	Biochain	10x10 µg	EUR 2199

cDNA - Liver Cirrhosis: Liver
C1236149Lcs	Biochain	40 reactions	EUR 729

Rat Liver Tissue Lysate
LYSATE0003	BosterBio	200ug	EUR 180
Description: This cell lysate is prepared from rat liver tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Liver Liver Cirrhosis Lysate
XBL-10358	ProSci	0.1 mg	EUR 796.2
Description: Human liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human liver tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Total RNA - Liver Cirrhosis: Liver
R1236149Lcs-50	Biochain	50 ug	EUR 421.2

Total Protein - Liver Cirrhosis: Liver
P1236149Lcs	Biochain	1 mg	EUR 471

Rat Ferritin (Liver) Antibody
11901-05011	AssayPro	150 ug	EUR 260.4

Rat Liver PrimaCell: Hepatocytes
2-82595	CHI Scientific	1 Kit	Ask for price

Liver Membrane Liver Cirrhosis Lysate
XBL-10671	ProSci	0.1 mg	EUR 752.1
Description: Human liver tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human liver tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated liver tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated liver tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Liver glycogen ELISA kit
E02H0052-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit
E02H0052-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit
E02H0052-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Membrane Protein - Liver Cirrhosis: Liver
P3236149Lcs	Biochain	0.1 mg	EUR 410

Rat Liver Whole tissue lysate
RAL-1464	Alpha Diagnostics	1 mg	EUR 628.8

Liver cancer and liver tissue array
BC03116a	TissueArray	each	EUR 306
Description: Liver cancer and liver tissue array, including pathology grade, TNM and clinical stage, 70 cases/70 cores, replacing BC03116

Liver tumor with liver tissue array
T032d	TissueArray	each	EUR 48
Description: Liver tumor with liver tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T032c

Liver cancer with liver tissue array
LV1021a	TissueArray	each	EUR 204
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 102 cases/102 cores, replacing LV1021

Liver cancer with liver tissue array
LV243	TissueArray	each	EUR 66
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores

Liver cancer with liver tissue array
LV722	TissueArray	each	EUR 306
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing LV721

Liver cancer with liver tissue array
LV803b	TissueArray	each	EUR 270
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 54 cases/72 cores, replacing LV803a

cDNA - Rat Normal Tissue: Liver
C1434149	Biochain	40 reactions	EUR 385

Paraffin Tissue Section - Liver Cirrhosis: Liver
T2236149Lcs	Biochain	5 slides	EUR 238

Liver cancer and normal liver tissue array
LV483a	TissueArray	each	EUR 198
Description: Liver cancer and normal liver tissue array, including hepatocellular carcinoma,cholangiocellular carcinoma and normal tissues pathology grade, TNM and clinical stage (AJCC 7th edition), 48 cases/48 cores (core size 1.5mm), replacing LV483

Liver cancer with matched liver tissue array
LV241b	TissueArray	each	EUR 66
Description: Liver cancer with matched liver tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing LV241a

Multiple liver disease with liver tissue array
LV601	TissueArray	each	EUR 306
Description: Multiple liver disease with liver tissue array, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 46 cases/60 cores

Rat Collectin Liver 1 ELISA kit
E02C0405-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit
E02C0405-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit
E02C0405-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA - Rat Normal Tissue: Liver
R1434149-50	Biochain	50 ug	EUR 130

Liver Lysate
1464	ProSci	0.1 mg	EUR 229.2
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate
21-105	ProSci	0.1 mg	EUR 342.6
Description: Bovine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Liver Lysate
21-406	ProSci	0.1 mg	EUR 342.6
Description: Porcine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Liver Lysate
21-419	ProSci	0.1 mg	EUR 342.6
Description: Rabbit liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Liver cancer
BC03117	TissueArray	each	EUR 306
Description: Liver cancer, liver cirrhosis tissue array with normal tissue as control, including TNM, clinical stage, pathology grade and history of hepatitis, 70 cases/ 80 cores, replaced by BC03117a

Liver tissue
LV805b	TissueArray	each	EUR 270
Description: Liver tissue, hepatitis and cirrhosis tissue array, 80 cases/80 cores, replacing LV805a

Hepatitis B liver cancer and liver tissue array
LV1401	TissueArray	each	EUR 474
Description: Hepatitis B liver cancer and liver tissue array, including pathology grade, TNM, clinical stage and HBV data, 99 cases/140 cores

Rat Liver specific lipootein ELISA kit
E02L0280-192T	BlueGene	192 tests	EUR 1524

Description: A sandwich ELISA for quantitative measurement of Rat Liver specific lipootein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver specific lipootein ELISA kit
E02L0280-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A sandwich ELISA for quantitative measurement of Rat Liver specific lipootein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver specific lipootein ELISA kit
E02L0280-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A sandwich ELISA for quantitative measurement of Rat Liver specific lipootein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat FibrOut 2, for cartilage, liver
4-20529	CHI Scientific	1 ml	Ask for price

Rat FibrOut 2, for cartilage, liver
4-20530	CHI Scientific	5 x 1 ml	Ask for price

High density liver cancer with liver tissue array
LV208	TissueArray	each	EUR 546
Description: High density liver cancer with liver tissue array, including pathology grade, TNM and clinical stage., 69 cases/207 cores

High density liver cancer with liver tissue array
LV2091	TissueArray	each	EUR 474
Description: High density liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 208 cases/208 cores

Liver Peptone
MED1508	Scientific Laboratory Supplies	EACH	EUR 86.17

Rat liver ferritin purified, ELISA grade
FERT21-R-50	Alpha Diagnostics	50 ug	EUR 489.6

Genomic DNA - Rat Normal Tissue: Liver
D1434149	Biochain	100 ug	EUR 286

Rat Liver X receptor alpha ELISA kit
E02L0342-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Liver X receptor alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver X receptor alpha ELISA kit
E02L0342-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rat Liver X receptor alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver X receptor alpha ELISA kit
E02L0342-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rat Liver X receptor alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Liver cancer and normal adjacent liver tissue array
LV801b	TissueArray	each	EUR 306
Description: Liver cancer and normal adjacent liver tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores (1.5mm), replacing LV801a

Liver cancer with adjacent normal liver tissue array
LV242a	TissueArray	each	EUR 66
Description: Liver cancer with adjacent normal liver tissue array, including pathology grade, TNM and clinical stage (reference AJCC 7th version), 12 cases/24 cores, replacing LV242

CK LIVER 1kg*
43023-2	Pel-Freez	1 kg	EUR 288.11

Rat Arginase 1 (Liver type) ELISA kit
E02A0544-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Arginase 1 (Liver type) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Arginase 1 (Liver type) ELISA kit
E02A0544-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rat Arginase 1 (Liver type) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Arginase 1 (Liver type) ELISA kit
E02A0544-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rat Arginase 1 (Liver type) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Genomic DNA - Liver Cirrhosis: Liver, from a single donor
D1236149Lcs	Biochain	50 ug	EUR 489

Rat Phosphofructokinase, Liver (PFKL) Protein
20-abx167625	Abbexa	EUR 844.80 EUR 343.20 EUR 2598.00 EUR 994.80 EUR 594.00	100 ug 10 ug 1 mg 200 ug 50 ug

Different stages of liver cancer and liver tissue array
LV808	TissueArray	each	EUR 306
Description: Different stages of liver cancer and liver tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores

Rat Liver OptiTDS: Tissue Dissociation System
4-28322	CHI Scientific	1 Kit	Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat
4-20304	CHI Scientific	ea	Ask for price

Rat Liver Receptor Homolog 1 (LRH1) Protein
20-abx650553	Abbexa	EUR 844.80 EUR 343.20 EUR 2598.00 EUR 994.80 EUR 594.00	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Liver X Receptor Beta (LXRb) ELISA Kit
abx354223-96tests	Abbexa	96 tests	EUR 943.2

Rat Liver X receptor α(LXR-αELISA Kit
QY-E10597	Qayee Biotechnology	96T	EUR 480

Rat Soluble Liver X Receptor ELISA kit
E02L0297-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Soluble Liver X Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Soluble Liver X Receptor ELISA kit
E02L0297-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rat Soluble Liver X Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Soluble Liver X Receptor ELISA kit
E02L0297-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rat Soluble Liver X Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Liver CAFs
CAF113	Neuromics	1,000,000 cells	Ask for price

Chang Liver (HeLa)
C0050007	Addexbio	One Frozen vial	EUR 651.6

cDNA - Lupus: Liver
C1236149Lup	Biochain	40 reactions	EUR 737

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
DLR-LXRa-Ra-48T	DL Develop	48T	EUR 658.8

Description: A sandwich quantitative ELISA assay kit for detection of Rat Liver X Receptor Alpha (LXRa) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
DLR-LXRa-Ra-96T	DL Develop	96T	EUR 861.6

Description: A sandwich quantitative ELISA assay kit for detection of Rat Liver X Receptor Alpha (LXRa) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
abx354222-96tests	Abbexa	96 tests	EUR 943.2

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
RD-LXRa-Ra-48Tests	Reddot Biotech	48 Tests	EUR 668.4

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
RD-LXRa-Ra-96Tests	Reddot Biotech	96 Tests	EUR 930

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
RDR-LXRa-Ra-48Tests	Reddot Biotech	48 Tests	EUR 699.6

Rat Liver X Receptor Alpha (LXRa) ELISA Kit
RDR-LXRa-Ra-96Tests	Reddot Biotech	96 Tests	EUR 973.2

Liver cancer with matched cancer adjacent liver tissue array
LV1505a	TissueArray	each	EUR 258
Description: Liver cancer with matched cancer adjacent liver tissue array, including pathology grade, TNM and clinical stage, replacing LV1505

Rat Glycogen Phosphorylase, Liver ELISA kit
E02P0171-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Glycogen Phosphorylase, Liver in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glycogen Phosphorylase, Liver ELISA kit
E02P0171-48	BlueGene	1 plate of 48 wells	EUR 624

Description: A competitive ELISA for quantitative measurement of Rat Glycogen Phosphorylase, Liver in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glycogen Phosphorylase, Liver ELISA kit
E02P0171-96	BlueGene	1 plate of 96 wells	EUR 822

Description: A competitive ELISA for quantitative measurement of Rat Glycogen Phosphorylase, Liver in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

PG LIVER 2 EA*
59423-2	Pel-Freez	2 EA	EUR 230.69

Rat Anti soluble liver antigen ELISA kit
E02S0263-192T	BlueGene	192 tests	EUR 1524

Description: A competitive ELISA for quantitative measurement of Rat Anti soluble liver antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

N-Glucuronide and sequential TZD ring-opened metabolites have been additionally present in liver microsomes within the presence of UDP-glucuronic acid. The O-demethyl-O-sulfate (M11), which is the main in vivo metabolite in rats and monkeys, was detected in all species of hepatocytes. As well as, a TZD ring-opened S-cysteine conjugate (M15) was detected in human hepatocytes. From these outcomes, the in vivo metabolic pathways in people have been predicted to be the 4 oxidation and one N-glucuronidation pathways. The 4 oxidative metabolites have been fashioned by a number of human P450 enzymes, and N-glucuronide was fashioned by UGT1A3 and UGT2B7.

In vitro metabolism of BIIB021, an inhibitor of warmth shock protein 90, in liver microsomes and hepatocytes of rats, canines, and people and recombinant human cytochrome P450 isoforms.

In vitro metabolism of rivoglitazone, a novel peroxisome proliferator-activated receptor γ agonist, in rat, monkey, and human liver microsomes and freshly remoted hepatocytes.

Kinesis Adaptor Body 1/2-20 Female x 1/4-28 Female

Kinesis Adapter Luer Female to M6 Female; Black PEEK

Control Genomic DNA - Rat Female

Female FPLC Connector

Female Nut BSPP R/H

C-FLEX TUBING, 3'- 1/4" ID, WITH FEMALE MPC CONNECTOR TO FEMALE LUER W/CAP

Female Nut BSPP L/H 6mm

Kinesis Female Luer Fittings

Control Genomic DNA - Dog Female

Control Genomic DNA - Human Female

Control Genomic DNA - Mouse Female

Control Genomic DNA - Horse Female

Kinesis Luer Female - 1/8in Barb

Kinesis Female Nut (Micro Fittings)

Control Genomic DNA - Rabbit Female

Control Genomic DNA - Bovine Female

Kinesis Luer Female - 3/32in Barb

Control Genomic DNA - Porcine Female

Control Genomic DNA - Chicken Female

Control Genomic DNA - Hamster Female

Kinesis Adapter SS 10.32 Female to 1/4-28 Male

Kinesis 10-32 Female to Male Luer Nut

Kinesis Adapter PEEK 10.32 Female to 1/4-28 Male

Kinesis Female Luer - M6 Tefzel Black

Neurons (iPSC from Blood Cells; Female)

Age grouped female skin tissue array

Kinesis Adaptor Body - 1/4-28 Female x 1/4-28 Male

Kinesis Siltite Fingertight Female Nut

Kinesis Adapter 1/2-20 Female to 1/4-28 Male; PCTFE

Control Genomic DNA - Guinea Pig Female

Kinesis Adapter Kel-F M6 Female to 1/4-28 Male

Kinesis Adaptor 5/16-24 Female To 1/4-28 Male; Kel-F

Kinesis Adapter 5/16-24 Female to 1/4-28 Male Kel-F

Female reproductive system tissue array

Kinesis Female Luer to MicroTight Body

V-Neck Polycotton Lab Coat - Female - 100cm/40in - Medium

Kinesis Adapter Body PEEK 10.32 Female to 1/4-28 Male

Kinesis Female Nut 5/16-24 PEEK Black

Kinesis Female Luer to 10-32 Male PEEK

Kinesis Adaptor PTFE 1/4-28 Female x 1/4-28 Male long

Kinesis Luer Assembly M6 Female to Male

Kinesis Adaptor 1/4-28 Female to M6 Male

Control Genomic DNA - Rhesus Monkey Female

Kinesis Female Luer Fitting System - 1/8

Kinesis Female Luer Fitting System - 1/16

Adapter X2 M30x1.5 female to NPT 3/4" male

Adapter X2 M30x1.5 female to NPT 3/4" male

Kinesis Adapter PEEK 10.32 Male to M6 Female

Kinesis Adapter PEEK 10.32 Female to M6 Male

Kinesis Adapter Male luer to 10-32 Female PEEK

Control Genomic DNA - Cynomolgus Monkey Female

Kinesis Adapter Male luer to M6 Female ETFE

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female

Kinesis Female Nut PEEK Black 1/4-28 - 1/8

Female reproductive system tumor tissue array

Kinesis Adapter Male Luer to 1/4-28 Female PEEK

Kinesis Adapter Male luer to 1/4-28 Female ETFE

Astrocytes Mature (iPSC from Blood Cells; Female)

Normal female dog multiple organs tissue array

Neural Stem Cells (iPSC from Blood Cells; Female)

Kinesis Female Nut PEEK Nat for 1/16in Tubing

Kinesis Adapter Female luer to Male 10-32 ETFE

Kinesis Adapter Female Luer to Male 1/4-28; PP

Kinesis Adapter PEEK 10.32 Flat Male to 1/4-28 Female

Neurons Starter Kit (iPSC from Blood Cells; Female)

Tubing 1mMetal insulated 2 fittings M16x1 female

Dopaminergic Neurons (iPSC from Blood Cells; Female)

Kinesis Adapter Female Luer to Male 1/4-28; ETFE

Kinesis Adaptor; Female Luer to 5/16-24 Male; ETFE

Kinesis Adapter PP; Barb 1/16 to Luer Lock Female

Astrocytes Precursors (iPSC from Blood Cells; Female)

Kinesis Adaptor 5/16-24 Male To 1/4-28 Female; Kel-F

Kinesis Adapter Female Luer to Male 1/4-28; Delrin

V-Neck Polycotton Lab Coat - Female - 92cm/36in - Small

V-Neck Polycotton Lab Coat - Female - 96cm/38in - Medium

Multiplex ELISA Kit For Human Female Hormone - (8-plex)

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female; Extra Long