In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites.

In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites. post thumbnail image

Within the current examine, the in vitro metabolic habits of a benzopyridooxathiazepine (BZN), a potent tubulin polymerization inhibitor, was investigated by liquid chromatography-UV detection (LC-UV). First, easy and quick LC-UV strategies have been optimized and validated to judge the pharmacokinetic profile of BZN utilizing rat liver microsomes or hepatocytes main cultures suspensions. Regardless of the medium investigated, baseline decision between the interior commonplace and BZN was achieved in a run time lower than 15min utilizing a Symmetry ODS column (150mm×4.6mm i.d., 5μm) and a cell part consisting of acetonitrile/water/formic acid 60:40:0.1 (v/v/v). Linearity was assessed within the 0.1-50μM and within the 0.05-5μM focus ranges, respectively, in microsomal and hepatocyte matrix.

Based on the novel technique based mostly on the construct of the accuracy profile, whole error of the developed strategies was included inside the ±10% limits of acceptance. Then, from incubation of BZN with each liver microsomes and or hepatocytes, structural informations on part I and part II metabolites have been acquired utilizing liquid chromatography coupled to electrospray orbitrap mass spectrometer (LC-MS). Mass spectrum, double bond equal and elemental composition have been helpful knowledge to entry to the chemical construction of every metabolite.

In microsomal suspension, 4 predominant metabolites have been noticed together with monohydroxylation and dihydroxylation of the benzopyridooxathiazepine core, demethylation of the methoxyphenyl moiety, in addition to their mixtures. The part II metabolites detected in hepatocytes suspension have been the glucuronide adducts of each demethylated BZN and mono-oxygenated BZN. Primarily based on the structural elucidation of the metabolites detected, we proposed an in vitro metabolic pathway of BZN, a brand new tubulin polymerization inhibitor.

In vitro metabolism of BIIB021, an inhibitor of warmth shock protein 90, in liver microsomes and hepatocytes of rats, canines, and people and recombinant human cytochrome P450 isoforms.

Inhibition of warmth shock protein 90 (HSP90) ends in the degradation of oncoproteins that drive malignant development and induce cell loss of life, thus making HSP90 a possible goal of most cancers remedy. 6-Chloro-9-(4-methoxy-3, 5-dimethyl-pyridin-2-ylmethyl)-9H-purin-2-ylamine (BIIB021), an artificial HSP90 inhibitor, exhibited promising antitumor exercise in preclinical fashions. It’s at present in part II medical trials for the oral remedy of breast most cancers. The target of this examine was to acquire each quantitative and qualitative metabolic profiles of [(14)C]BIIB021 in rat, canine, and human liver microsomes and hepatocytes to supply assist for in vivo security and medical research.

The metabolites of [(14)C]BIIB021 have been recognized utilizing liquid chromatography-tandem mass spectrometry coupled with radiometric detection. BIIB021 was extensively metabolized in each liver microsomes and hepatocytes. The foremost oxidative metabolic pathways recognized for all species have been attributable to hydroxylation (M7) and O-demethylation (M2) of the methoxy-dimethylpyridine moiety. The vast majority of M7 in canine hepatocytes was additional conjugated to type the glucuronide (M4). Oxidative dechlorination (M6), monooxygenation (M10), and oxidative N-dealkylation of the methoxy-dimethylpyridine moiety (M11 and M12) have been noticed because the minor metabolic pathways in hepatocytes of all three species. A glutathione conjugate (M18) was additionally recognized in all species.

In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites.

Its formation was catalyzed, partially, by soluble glutathione transferase by way of direct displacement of the chlorine on the amino-chloropurine moiety. Subsequent minor secondary metabolites M13, M14, M15, and M17 have been noticed in human, canine, and rat hepatocytes. Outcomes from incubations of BIIB021 with human recombinant cytochrome P450 (P450) isoforms and a P450 antibody inhibition examine in human liver microsomes urged that the formation of M7 is especially catalyzed by CYP2C19 and CYP3A4, whereas the formation of minor metabolite M2 in human liver microsomes most likely may very well be attributed to CYP3A4.

In vitro metabolism of rivoglitazone, a novel peroxisome proliferator-activated receptor γ agonist, in rat, monkey, and human liver microsomes and freshly remoted hepatocytes.

The in vitro metabolism of rivoglitazone, (RS)-5-{4-[(6-methoxy-1-methyl-1H-benzimidazol-2-yl)methoxy]benzyl}-1,3-thiazolidine-2,4-dione monohydrochloride, a novel thiazolidinedione (TZD) peroxisome proliferator-activated receptor γ selective agonist, was studied in liver microsomes and freshly remoted hepatocytes of rat, monkey, and human in addition to cDNA-expressed human cytochrome P450 (P450) and UDP-glucuronosyltransferase (UGT) enzymes. Fourteen metabolites have been detected, and these constructions have been elucidated by liquid chromatography-tandem mass spectrometry.

5 preliminary metabolic pathways of rivoglitazone consisting of 4 oxidation pathways and one N-glucuronidation pathway have been predicted in correspondence with these proposed for in vivo research utilizing rats and monkeys. In metabolization utilizing liver microsomes, the TZD ring-opened mercapto amide (M22) and TZD ring-opened mercapto carboxylic acid (M23) have been recognized as the first metabolite of the TZD ring-opening pathway and its sequential metabolite, which haven’t been detected beforehand from in vivo research. Mixture with S-adenosyl-L-methionine was helpful to acquire the sequential S-methylated metabolites from the oxidative metabolites.

Kinesis Adaptor Body 1/2-20 Female x 1/4-28 Female

CHR5105 EACH
EUR 55.2

Kinesis Adapter Luer Female to M6 Female; Black PEEK

CHR4317 EACH
EUR 22.8

Control Genomic DNA - Rat Female

D1434999-G02 100 ug
EUR 170

Female FPLC Connector

FFC-1 1/pk
EUR 93.6

Female Nut BSPP R/H

GAS1086 EACH
EUR 23.14

C-FLEX TUBING, 3'- 1/4" ID, WITH FEMALE MPC CONNECTOR TO FEMALE LUER W/CAP

91-700-04 1/pk
EUR 82.8
Description: Flexible Containers; Bioprocess Bags and Parts

Female Nut BSPP L/H 6mm

GAS1092 EACH
EUR 15.34

Kinesis Female Luer Fittings

CHR1247 PK2
EUR 7.2

Control Genomic DNA - Dog Female

D1734999-G02 100 ug
EUR 170

Control Genomic DNA - Human Female

D1234999-G02 100 ug
EUR 170

Control Genomic DNA - Mouse Female

D1334999-G02 100 ug
EUR 170

Control Genomic DNA - Horse Female

D1O34999-G02 100 ug
EUR 170

Kinesis Luer Female - 1/8in Barb

CHR4593 EACH
EUR 2.4

Kinesis Female Nut (Micro Fittings)

CHR4137 EACH
EUR 12

Control Genomic DNA - Rabbit Female

D1834999-G02 100 ug
EUR 170

Control Genomic DNA - Bovine Female

D1B34999-G02 100 ug
EUR 170

Kinesis Luer Female - 3/32in Barb

CHR4591 EACH
EUR 3.6

Control Genomic DNA - Porcine Female

D1934999-G02 100 ug
EUR 170

Control Genomic DNA - Chicken Female

D1C34999-G02 100 ug
EUR 170

Control Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 170

Kinesis Adapter SS 10.32 Female to 1/4-28 Male

CHR2347 EACH
EUR 36

Kinesis 10-32 Female to Male Luer Nut

CHR4311 EACH
EUR 16.8

Kinesis Adapter PEEK 10.32 Female to 1/4-28 Male

CHR4333 EACH
EUR 38.4

Kinesis Female Luer - M6 Tefzel Black

CHR4363 EACH
EUR 8.4

Neurons (iPSC from Blood Cells; Female)

ASE-9321F 1 vial (1 x 10^6)
EUR 375.9
Description: 12 month

Age grouped female skin tissue array

SK244a each
EUR 270
Description: Age grouped female skin tissue array, 12 cases/24 cores, replacing SK244

Kinesis Adaptor Body - 1/4-28 Female x 1/4-28 Male

CHR4339 EACH
EUR 51.6

Kinesis Siltite Fingertight Female Nut

CHR1941 PK5
EUR 136.8

Kinesis Adapter 1/2-20 Female to 1/4-28 Male; PCTFE

CHR4361 EACH
EUR 39.6

Control Genomic DNA - Guinea Pig Female

D1G34999-G02 100 ug
EUR 170

Kinesis Adapter Kel-F M6 Female to 1/4-28 Male

CHR4337 EACH
EUR 39.6

Kinesis Adaptor 5/16-24 Female To 1/4-28 Male; Kel-F

CHR4299 EACH
EUR 44.4

Kinesis Adapter 5/16-24 Female to 1/4-28 Male Kel-F

CHR4355 EACH
EUR 33.6

Female reproductive system tissue array

FRS801 each
EUR 306
Description: Female reproductive system tissue array, with stage and grade info, 80 cases/80 cores

Kinesis Female Luer to MicroTight Body

CHR4323 EACH
EUR 75.6

V-Neck Polycotton Lab Coat - Female - 100cm/40in - Medium

SAF0078 EACH
EUR 61.2

Kinesis Adapter Body PEEK 10.32 Female to 1/4-28 Male

CHR4335 EACH
EUR 33.6

Kinesis Female Nut 5/16-24 PEEK Black

CHR4139 EACH
EUR 12

Kinesis Female Luer to 10-32 Male PEEK

CHR4419 EACH
EUR 46.8

Kinesis Adaptor PTFE 1/4-28 Female x 1/4-28 Male long

CHR4373 EACH
EUR 43.2

Kinesis Luer Assembly M6 Female to Male

CHR4313 EACH
EUR 24

Kinesis Adaptor 1/4-28 Female to M6 Male

CHR4375 EACH
EUR 40.8

Control Genomic DNA - Rhesus Monkey Female

D1534999-G02 100 ug
EUR 170

Kinesis Female Luer Fitting System - 1/8

CHR4545 EACH
EUR 7.2

Kinesis Female Luer Fitting System - 1/16

CHR4553 EACH
EUR 7.2

Adapter X2 M30x1.5 female to NPT 3/4" male

8890035 PK2
EUR 169.2

Adapter X2 M30x1.5 female to NPT 3/4" male

8890083 PK2
EUR 258

Kinesis Adapter PEEK 10.32 Male to M6 Female

CHR4301 EACH
EUR 22.8

Kinesis Adapter PEEK 10.32 Female to M6 Male

CHR4633 EACH
EUR 39.6

Kinesis Adapter Male luer to 10-32 Female PEEK

CHR4309 EACH
EUR 21.6

Control Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 100 ug
EUR 170

Kinesis Adapter Male luer to M6 Female ETFE

CHR4351 EACH
EUR 16.8

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female

CHR4303 EACH
EUR 22.8

Kinesis Female Nut PEEK Black 1/4-28 - 1/8

CHR3357 EACH
EUR 12

Female reproductive system tumor tissue array

FE951 each
EUR 354
Description: Female reproductive system tumor tissue array, including pathology grade, TNM and clinical stage (AJCC 8.0), 95 cases/95 cores (core size 1.5mm), Recent Year Collection

Kinesis Adapter Male Luer to 1/4-28 Female PEEK

CHR4307 EACH
EUR 21.6

Kinesis Adapter Male luer to 1/4-28 Female ETFE

CHR4347 EACH
EUR 14.4

Astrocytes Mature (iPSC from Blood Cells; Female)

ASE-9322MF 1 vial (1 x 10^6)
EUR 546
Description: 12 month

Normal female dog multiple organs tissue array

DGF281 each
EUR 306
Description: Normal female dog multiple organs tissue array, 28 organs/ 28cores

Neural Stem Cells (iPSC from Blood Cells; Female)

ASE-9234F 1 vial (1 x 10^6)
EUR 497.4
Description: 12 month

Kinesis Female Nut PEEK Nat for 1/16in Tubing

CHR4141 EACH
EUR 13.2

Kinesis Adapter Female luer to Male 10-32 ETFE

CHR4287 EACH
EUR 8.4

Kinesis Adapter Female Luer to Male 1/4-28; PP

CHR4241 EACH
EUR 8.4

Kinesis Adapter PEEK 10.32 Flat Male to 1/4-28 Female

CHR4341 EACH
EUR 39.6

Neurons Starter Kit (iPSC from Blood Cells; Female)

ASE-9321KF 1 Kit
EUR 473.1
Description: 12 month

Tubing 1mMetal insulated 2 fittings M16x1 female

8930221 KIT
EUR 340.8

Dopaminergic Neurons (iPSC from Blood Cells; Female)

ASE-9323F 1 vial
EUR 448.8
Description: 12 month

Kinesis Adapter Female Luer to Male 1/4-28; ETFE

CHR4255 EACH
EUR 8.4

Kinesis Adaptor; Female Luer to 5/16-24 Male; ETFE

CHR4319 EACH
EUR 8.4

Kinesis Adapter PP; Barb 1/16 to Luer Lock Female

CHR4589 EACH
EUR 2.4

Astrocytes Precursors (iPSC from Blood Cells; Female)

ASE-9322PF 1 vial (1 x 10^6)
EUR 448.8
Description: 12 month

Kinesis Adaptor 5/16-24 Male To 1/4-28 Female; Kel-F

CHR4417 EACH
EUR 39.6

Kinesis Adapter Female Luer to Male 1/4-28; Delrin

CHR4231 EACH
EUR 7.2

V-Neck Polycotton Lab Coat - Female - 92cm/36in - Small

SAF0074 EACH
EUR 62.4

V-Neck Polycotton Lab Coat - Female - 96cm/38in - Medium

SAF0076 EACH
EUR 60

Multiplex ELISA Kit For Human Female Hormone - (8-plex)

MEK1022 1 kit
EUR 1696.8

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female; Extra Long

CHR4305 EACH
EUR 25.2

Kinesis Adapter Assembly 1/8 NPT x 1/4-28 Female PEEK Red

CHR5065 EACH
EUR 25.2

Kinesis Adapter Female Luer to Male 1/4-28; PP; Large Bore

CHR4243 EACH
EUR 8.4

Kinesis Female Luer to Barb Adapter 1/16ID Tubing; Tefzel

CHR4605 EACH
EUR 7.2

Kinesis Adapter Assembly 1/4in NPT x 1/4-28 Female PEEK Red

CHR5053 EACH
EUR 30

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female)

ASE-9323KF 1 vial
EUR 546
Description: 12 month

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female)

ASE-9322PKF 1 Kit
EUR 497.4
Description: 12 month

C-FLEX TUBING, 3'- 1/4" ID, WITH MALE LUER WITH CAP TO FEMALE LUER

91-700-00 1/pk
EUR 79.2
Description: Flexible Containers; Bioprocess Bags and Parts

CELLSTACK,FILLING ACCESSORY,3/8 IN C-FLEX,FEMALE MPC,S,1/4

11902 1/pk
EUR 337.2
Description: Stackable Cell Culture; Closed Systems Cell Culture Chambers

Monkey Capuchin (Cebus) control (non-immunized) serum, Male and Female

NMCB-1 0.5 ml
EUR 196.8

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-5 5 slides
EUR 883

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I

T6834700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I

T6834700-G02-5 5 slides
EUR 883

Elbow fittings x2 90° M16x1 female/male stainless steel side length 2x54mm

8970442 PK2
EUR 262.8

CELLSTACK,FILLING ACC,33MM CAP,MPC FEMALE QK CON,W/DUSTCVR,3/8"ID,S,IND,1/4

3329 1/pk
EUR 337.2
Description: Stackable Cell Culture; CellSTACK

A-431 Cell Slide (Human (85 yrs, Female) skin epidermoid carcinoma) (5 slides/pk)

HCLS-17002 1 pk
EUR 300

HeLa Cell Slide (Human (31 yrs, Female) cervix epithelioid carcinoma) (5 slides/pk)

HCLS-17001 1 pk
EUR 300

Option quick disconnect kit, includes 2 male/female attachments & silicone tubing

V0020-QD 1 PC
EUR 144.9

Monkey Marmoset (Callithrix jacchus) control (non-immunized) serum, Male and Female

NMMS-1 0.5 ml
EUR 270

Option quick disconnect kit, includes 2 male/female attachments and silicone tubing

BCM1550 1 pcs, 1 UNIT
EUR 244.87

L1210 Cell Slide (DBA subline 212 Mouse (8 months, Female) lymphocytic leukemia) (5 slides/pk)

MCLS-17204 1 pk
EUR 300

Monkey African Green (Chlorocebus) control (non-immunized) serum, Male and Female Available

NMAG-1 1 ml
EUR 169.2

Corning 33mm Filling Cap 1/4inch (6.4mm) ID Tubing a Male MPC Coupling and a Female MPC End Cap

3333 PK4
EUR 355.2

Corning 33mm Filling Cap with a Female MPC with a 1/4inch (6.4mm) ID Coupling and a Male MPC End Cap

3328 PK4
EUR 333.6

MCF7 Cell Slide (Human (69 yrs, Female) mammary gland, breast, epithelial adenocarcinoma) (5 slides/pk)

HCLS-17012 1 pk
EUR 300

K-562 Cell Slide (Human (53yrs, Female) bone marrow, chronic myelogenous leukemia (CML)) (5 slides/pk)

HCLS-17004 1 pk
EUR 300

C-FLEX TUBING, 2'-1/4" ID, WITH FEMALE MPC CONNECTOR WITH 1/4" BARB WITH PINCH CLAMP AND FILLING BELL

91-700-12 1/pk
EUR 231.6
Description: Flexible Containers; Bioprocess Bags and Parts

Corning 33mm Filling Cap 1/8inch ID Tubing and a Female Luer 1/8inch Hose Barb a Male Luer Lock Plug

3282 PK5
EUR 267.6

HL-60 Cell Slide (Human (36yrs, Female) peripheral blood promyeloblast, acute promyelocytic leukemia) (5 slides/pk)

HCLS-17009 1 pk
EUR 300

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from 4 different donors, 2 male and 2 female. 1 ug/ul concentration

R1234148-10-P04 10x10 µg
EUR 2199

cDNA - Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 729

Rat Liver Tissue Lysate

LYSATE0003 200ug
EUR 180
Description: This cell lysate is prepared from rat liver tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Liver Liver Cirrhosis Lysate

XBL-10358 0.1 mg
EUR 796.2
Description: Human liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human liver tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Total RNA - Liver Cirrhosis: Liver

R1236149Lcs-50 50 ug
EUR 421.2

Total Protein - Liver Cirrhosis: Liver

P1236149Lcs 1 mg
EUR 471

Rat Ferritin (Liver) Antibody

11901-05011 150 ug
EUR 260.4

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Liver Membrane Liver Cirrhosis Lysate

XBL-10671 0.1 mg
EUR 752.1
Description: Human liver tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human liver tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated liver tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated liver tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Liver glycogen ELISA kit

E02H0052-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit

E02H0052-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit

E02H0052-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Membrane Protein - Liver Cirrhosis: Liver

P3236149Lcs 0.1 mg
EUR 410

Rat Liver Whole tissue lysate

RAL-1464 1 mg
EUR 628.8

Liver cancer and liver tissue array

BC03116a each
EUR 306
Description: Liver cancer and liver tissue array, including pathology grade, TNM and clinical stage, 70 cases/70 cores, replacing BC03116

Liver tumor with liver tissue array

T032d each
EUR 48
Description: Liver tumor with liver tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T032c

Liver cancer with liver tissue array

LV1021a each
EUR 204
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 102 cases/102 cores, replacing LV1021

Liver cancer with liver tissue array

LV243 each
EUR 66
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores

Liver cancer with liver tissue array

LV722 each
EUR 306
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing LV721

Liver cancer with liver tissue array

LV803b each
EUR 270
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 54 cases/72 cores, replacing LV803a

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 385

Paraffin Tissue Section - Liver Cirrhosis: Liver

T2236149Lcs 5 slides
EUR 238

Liver cancer and normal liver tissue array

LV483a each
EUR 198
Description: Liver cancer and normal liver tissue array, including hepatocellular carcinoma,cholangiocellular carcinoma and normal tissues pathology grade, TNM and clinical stage (AJCC 7th edition), 48 cases/48 cores (core size 1.5mm), replacing LV483

Liver cancer with matched liver tissue array

LV241b each
EUR 66
Description: Liver cancer with matched liver tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing LV241a

Multiple liver disease with liver tissue array

LV601 each
EUR 306
Description: Multiple liver disease with liver tissue array, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 46 cases/60 cores

Rat Collectin Liver 1 ELISA kit

E02C0405-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit

E02C0405-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit

E02C0405-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total RNA - Rat Normal Tissue: Liver

R1434149-50 50 ug
EUR 130

Liver Lysate

1464 0.1 mg
EUR 229.2
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate

21-105 0.1 mg
EUR 342.6
Description: Bovine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Liver Lysate

21-406 0.1 mg
EUR 342.6
Description: Porcine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Liver Lysate

21-419 0.1 mg
EUR 342.6
Description: Rabbit liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Liver cancer

BC03117 each
EUR 306
Description: Liver cancer, liver cirrhosis tissue array with normal tissue as control, including TNM, clinical stage, pathology grade and history of hepatitis, 70 cases/ 80 cores, replaced by BC03117a

Liver tissue

LV805b each
EUR 270
Description: Liver tissue, hepatitis and cirrhosis tissue array, 80 cases/80 cores, replacing LV805a

Hepatitis B liver cancer and liver tissue array

LV1401 each
EUR 474
Description: Hepatitis B liver cancer and liver tissue array, including pathology grade, TNM, clinical stage and HBV data, 99 cases/140 cores

Rat Liver specific lipootein ELISA kit

E02L0280-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Liver specific lipootein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver specific lipootein ELISA kit

E02L0280-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Liver specific lipootein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver specific lipootein ELISA kit

E02L0280-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Liver specific lipootein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat FibrOut 2, for cartilage, liver

4-20529 1 ml Ask for price

Rat FibrOut 2, for cartilage, liver

4-20530 5 x 1 ml Ask for price

High density liver cancer with liver tissue array

LV208 each
EUR 546
Description: High density liver cancer with liver tissue array, including pathology grade, TNM and clinical stage., 69 cases/207 cores

High density liver cancer with liver tissue array

LV2091 each
EUR 474
Description: High density liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 208 cases/208 cores

Liver Peptone

MED1508 EACH
EUR 86.17

Rat liver ferritin purified, ELISA grade

FERT21-R-50 50 ug
EUR 489.6

Genomic DNA - Rat Normal Tissue: Liver

D1434149 100 ug
EUR 286

Rat Liver X receptor alpha ELISA kit

E02L0342-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Liver X receptor alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver X receptor alpha ELISA kit

E02L0342-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Liver X receptor alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver X receptor alpha ELISA kit

E02L0342-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Liver X receptor alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Liver cancer and normal adjacent liver tissue array

LV801b each
EUR 306
Description: Liver cancer and normal adjacent liver tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores (1.5mm), replacing LV801a

Liver cancer with adjacent normal liver tissue array

LV242a each
EUR 66
Description: Liver cancer with adjacent normal liver tissue array, including pathology grade, TNM and clinical stage (reference AJCC 7th version), 12 cases/24 cores, replacing LV242

CK LIVER 1kg*

43023-2 1 kg
EUR 288.11

Rat Arginase 1 (Liver type) ELISA kit

E02A0544-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Arginase 1 (Liver type) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Arginase 1 (Liver type) ELISA kit

E02A0544-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Arginase 1 (Liver type) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Arginase 1 (Liver type) ELISA kit

E02A0544-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Arginase 1 (Liver type) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Genomic DNA - Liver Cirrhosis: Liver, from a single donor

D1236149Lcs 50 ug
EUR 489

Rat Phosphofructokinase, Liver (PFKL) Protein

20-abx167625
  • EUR 844.80
  • EUR 343.20
  • EUR 2598.00
  • EUR 994.80
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Different stages of liver cancer and liver tissue array

LV808 each
EUR 306
Description: Different stages of liver cancer and liver tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores

Rat Liver OptiTDS: Tissue Dissociation System

4-28322 1 Kit Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Rat Liver Receptor Homolog 1 (LRH1) Protein

20-abx650553
  • EUR 844.80
  • EUR 343.20
  • EUR 2598.00
  • EUR 994.80
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Liver X Receptor Beta (LXRb) ELISA Kit

abx354223-96tests 96 tests
EUR 943.2

Rat Liver X receptor α(LXR-αELISA Kit

QY-E10597 96T
EUR 480

Rat Soluble Liver X Receptor ELISA kit

E02L0297-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Soluble Liver X Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Soluble Liver X Receptor ELISA kit

E02L0297-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Soluble Liver X Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Soluble Liver X Receptor ELISA kit

E02L0297-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Soluble Liver X Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Liver CAFs

CAF113 1,000,000 cells Ask for price

Chang Liver (HeLa)

C0050007 One Frozen vial
EUR 651.6

cDNA - Lupus: Liver

C1236149Lup 40 reactions
EUR 737

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

DLR-LXRa-Ra-48T 48T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Liver X Receptor Alpha (LXRa) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

DLR-LXRa-Ra-96T 96T
EUR 861.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Liver X Receptor Alpha (LXRa) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

abx354222-96tests 96 tests
EUR 943.2

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

RD-LXRa-Ra-48Tests 48 Tests
EUR 668.4

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

RD-LXRa-Ra-96Tests 96 Tests
EUR 930

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

RDR-LXRa-Ra-48Tests 48 Tests
EUR 699.6

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

RDR-LXRa-Ra-96Tests 96 Tests
EUR 973.2

Liver cancer with matched cancer adjacent liver tissue array

LV1505a each
EUR 258
Description: Liver cancer with matched cancer adjacent liver tissue array, including pathology grade, TNM and clinical stage, replacing LV1505

Rat Glycogen Phosphorylase, Liver ELISA kit

E02P0171-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Glycogen Phosphorylase, Liver in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glycogen Phosphorylase, Liver ELISA kit

E02P0171-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Glycogen Phosphorylase, Liver in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glycogen Phosphorylase, Liver ELISA kit

E02P0171-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Glycogen Phosphorylase, Liver in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

PG LIVER 2 EA*

59423-2 2 EA
EUR 230.69

Rat Anti soluble liver antigen ELISA kit

E02S0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Anti soluble liver antigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

N-Glucuronide and sequential TZD ring-opened metabolites have been additionally present in liver microsomes within the presence of UDP-glucuronic acid. The O-demethyl-O-sulfate (M11), which is the main in vivo metabolite in rats and monkeys, was detected in all species of hepatocytes. As well as, a TZD ring-opened S-cysteine conjugate (M15) was detected in human hepatocytes. From these outcomes, the in vivo metabolic pathways in people have been predicted to be the 4 oxidation and one N-glucuronidation pathways. The 4 oxidative metabolites have been fashioned by a number of human P450 enzymes, and N-glucuronide was fashioned by UGT1A3 and UGT2B7.

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