In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites.

In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites. post thumbnail image

Within the current examine, the in vitro metabolic habits of a benzopyridooxathiazepine (BZN), a potent tubulin polymerization inhibitor, was investigated by liquid chromatography-UV detection (LC-UV). First, easy and quick LC-UV strategies have been optimized and validated to judge the pharmacokinetic profile of BZN utilizing rat liver microsomes or hepatocytes main cultures suspensions. Regardless of the medium investigated, baseline decision between the interior commonplace and BZN was achieved in a run time lower than 15min utilizing a Symmetry ODS column (150mm×4.6mm i.d., 5μm) and a cell part consisting of acetonitrile/water/formic acid 60:40:0.1 (v/v/v). Linearity was assessed within the 0.1-50μM and within the 0.05-5μM focus ranges, respectively, in microsomal and hepatocyte matrix.

Based on the novel technique based mostly on the construct of the accuracy profile, whole error of the developed strategies was included inside the ±10% limits of acceptance. Then, from incubation of BZN with each liver microsomes and or hepatocytes, structural informations on part I and part II metabolites have been acquired utilizing liquid chromatography coupled to electrospray orbitrap mass spectrometer (LC-MS). Mass spectrum, double bond equal and elemental composition have been helpful knowledge to entry to the chemical construction of every metabolite.

In microsomal suspension, 4 predominant metabolites have been noticed together with monohydroxylation and dihydroxylation of the benzopyridooxathiazepine core, demethylation of the methoxyphenyl moiety, in addition to their mixtures. The part II metabolites detected in hepatocytes suspension have been the glucuronide adducts of each demethylated BZN and mono-oxygenated BZN. Primarily based on the structural elucidation of the metabolites detected, we proposed an in vitro metabolic pathway of BZN, a brand new tubulin polymerization inhibitor.

In vitro metabolism of BIIB021, an inhibitor of warmth shock protein 90, in liver microsomes and hepatocytes of rats, canines, and people and recombinant human cytochrome P450 isoforms.

Inhibition of warmth shock protein 90 (HSP90) ends in the degradation of oncoproteins that drive malignant development and induce cell loss of life, thus making HSP90 a possible goal of most cancers remedy. 6-Chloro-9-(4-methoxy-3, 5-dimethyl-pyridin-2-ylmethyl)-9H-purin-2-ylamine (BIIB021), an artificial HSP90 inhibitor, exhibited promising antitumor exercise in preclinical fashions. It’s at present in part II medical trials for the oral remedy of breast most cancers. The target of this examine was to acquire each quantitative and qualitative metabolic profiles of [(14)C]BIIB021 in rat, canine, and human liver microsomes and hepatocytes to supply assist for in vivo security and medical research.

The metabolites of [(14)C]BIIB021 have been recognized utilizing liquid chromatography-tandem mass spectrometry coupled with radiometric detection. BIIB021 was extensively metabolized in each liver microsomes and hepatocytes. The foremost oxidative metabolic pathways recognized for all species have been attributable to hydroxylation (M7) and O-demethylation (M2) of the methoxy-dimethylpyridine moiety. The vast majority of M7 in canine hepatocytes was additional conjugated to type the glucuronide (M4). Oxidative dechlorination (M6), monooxygenation (M10), and oxidative N-dealkylation of the methoxy-dimethylpyridine moiety (M11 and M12) have been noticed because the minor metabolic pathways in hepatocytes of all three species. A glutathione conjugate (M18) was additionally recognized in all species.

In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites.

Its formation was catalyzed, partially, by soluble glutathione transferase by way of direct displacement of the chlorine on the amino-chloropurine moiety. Subsequent minor secondary metabolites M13, M14, M15, and M17 have been noticed in human, canine, and rat hepatocytes. Outcomes from incubations of BIIB021 with human recombinant cytochrome P450 (P450) isoforms and a P450 antibody inhibition examine in human liver microsomes urged that the formation of M7 is especially catalyzed by CYP2C19 and CYP3A4, whereas the formation of minor metabolite M2 in human liver microsomes most likely may very well be attributed to CYP3A4.

In vitro metabolism of rivoglitazone, a novel peroxisome proliferator-activated receptor γ agonist, in rat, monkey, and human liver microsomes and freshly remoted hepatocytes.

The in vitro metabolism of rivoglitazone, (RS)-5-{4-[(6-methoxy-1-methyl-1H-benzimidazol-2-yl)methoxy]benzyl}-1,3-thiazolidine-2,4-dione monohydrochloride, a novel thiazolidinedione (TZD) peroxisome proliferator-activated receptor γ selective agonist, was studied in liver microsomes and freshly remoted hepatocytes of rat, monkey, and human in addition to cDNA-expressed human cytochrome P450 (P450) and UDP-glucuronosyltransferase (UGT) enzymes. Fourteen metabolites have been detected, and these constructions have been elucidated by liquid chromatography-tandem mass spectrometry.

5 preliminary metabolic pathways of rivoglitazone consisting of 4 oxidation pathways and one N-glucuronidation pathway have been predicted in correspondence with these proposed for in vivo research utilizing rats and monkeys. In metabolization utilizing liver microsomes, the TZD ring-opened mercapto amide (M22) and TZD ring-opened mercapto carboxylic acid (M23) have been recognized as the first metabolite of the TZD ring-opening pathway and its sequential metabolite, which haven’t been detected beforehand from in vivo research. Mixture with S-adenosyl-L-methionine was helpful to acquire the sequential S-methylated metabolites from the oxidative metabolites.

Kinesis Adapter 5/16-24 Female to 1/2-20 Female PEEK

CHR5095 EACH
EUR 78

Kinesis Adaptor 5/16-24 Female To 1/4-28 Female; PEEK

CHR3793 EACH
EUR 27.6

Kinesis Adaptor Body 1/2-20 Female x 1/4-28 Female

CHR5105 EACH
EUR 55.2

*Rat Renin Substrate Plasma Female

IRTRENSPF10ML each
EUR 4211
Description: *Rat Renin Substrate Plasma Female

*Rat Renin Substrate Plasma Female

IRTRENSPF1ML each
EUR 528
Description: *Rat Renin Substrate Plasma Female

Female FPLC Connector

FFC-1 1/pk
EUR 93.6

Female Nut BSPP R/H

GAS1086 EACH
EUR 23.14

Kinesis Female Luer Fittings

CHR1247 PK2
EUR 7.2

C-FLEX TUBING, 3'- 1/4" ID, WITH FEMALE MPC CONNECTOR TO FEMALE LUER W/CAP

91-700-04 1/pk
EUR 82.8
Description: Flexible Containers; Bioprocess Bags and Parts

Female Nut BSPP L/H 6mm

GAS1092 EACH
EUR 15.34

Control Genomic DNA - Dog Female

D1734999-G02 100 ug
EUR 170

Control Genomic DNA - Mouse Female

D1334999-G02 100 ug
EUR 170

Control Genomic DNA - Horse Female

D1O34999-G02 100 ug
EUR 170

Control Genomic DNA - Human Female

D1234999-G02 100 ug
EUR 170

Control Genomic DNA - Rabbit Female

D1834999-G02 100 ug
EUR 170

Control Genomic DNA - Bovine Female

D1B34999-G02 100 ug
EUR 170

Kinesis Female Nut (Micro Fittings)

CHR4137 EACH
EUR 12

Control Genomic DNA - Porcine Female

D1934999-G02 100 ug
EUR 170

Control Genomic DNA - Chicken Female

D1C34999-G02 100 ug
EUR 170

Control Genomic DNA - Hamster Female

D1H34999-G02 100 ug
EUR 170

Kinesis Luer Female - 1/8in Barb

CHR4593 EACH
EUR 2.4

Kinesis Luer Female - 3/32in Barb

CHR4591 EACH
EUR 3.6

Human Mammary Epithelial Cells, Female

ABC-TC3692 1 vial Ask for price
Description: Human Mammary Epithelial Cells, when grown in LIMam Medium, provide an ideal serum-free culture model for many areas of research. Common uses of HMEC include the stages of breast cancer development, three dimensional culture and carcinogen screening.Cell Features:HMEC are cryopreserved as tertiary cells, e.g. cells are isolated from human mammary tissue and expanded three times in culture vessels before being harvested for cryopreservation.HMEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Kinesis Siltite Fingertight Female Nut

CHR1941 PK5
EUR 136.8

Kinesis Female Luer - M6 Tefzel Black

CHR4363 EACH
EUR 8.4

Female reproductive system tissue array

FRS801 each
EUR 306
Description: Female reproductive system tissue array, with stage and grade info, 80 cases/80 cores

Human Hepatocytes-Single Donor, Female

ABC-TC3647 1 vial Ask for price
Description: Gentaur's hepatocytes for suspension assays (HP1 Heps-s, single donor, female) provide an economic source of characterized human hepatocytes for use in short-term suspension assays to evaluate the metabolism of drug candidates. Gentaur's hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available.

Neurons (iPSC from Blood Cells; Female)

ASE-9321F 1 vial (1 x 10^6)
EUR 375.9
Description: 12 month

Age grouped female skin tissue array

SK244a each
EUR 270
Description: Age grouped female skin tissue array, 12 cases/24 cores, replacing SK244

Control Genomic DNA - Guinea Pig Female

D1G34999-G02 100 ug
EUR 170

Single Donor Human Female Rectal Swab

IRHUSRCTSWBFEM1 each
EUR 428
Description: Single Donor Human Female Rectal Swab

Kinesis Female Luer to MicroTight Body

CHR4323 EACH
EUR 75.6

Single Donor Human Female Fecal Matter

IRHUFSMFEM1 each
EUR 382
Description: Single Donor Human Female Fecal Matter

Single Donor Human Female Fecal Matter

IRHUFSMFEM5 each
EUR 3566
Description: Single Donor Human Female Fecal Matter

Kinesis 10-32 Female to Male Luer Nut

CHR4311 EACH
EUR 16.8

Kinesis Adapter SS 10.32 Female to 1/4-28 Male

CHR2347 EACH
EUR 36

Control Genomic DNA - Rhesus Monkey Female

D1534999-G02 100 ug
EUR 170

Single Donor Human Female Perianal Swabs

IRHUSPANSWBFEM1 each
EUR 763
Description: Single Donor Human Female Perianal Swabs

Single Donor Human Female Perianal Swabs

IRHUSPANSWBFEM5 each
EUR 3809
Description: Single Donor Human Female Perianal Swabs

Kinesis Adapter PEEK 10.32 Female to 1/4-28 Male

CHR4333 EACH
EUR 38.4

Kinesis Luer Assembly M6 Female to Male

CHR4313 EACH
EUR 24

Kinesis Female Luer Fitting System - 1/8

CHR4545 EACH
EUR 7.2

Kinesis Female Luer Fitting System - 1/16

CHR4553 EACH
EUR 7.2

Kinesis Female Nut 5/16-24 PEEK Black

CHR4139 EACH
EUR 12

Kinesis Female Luer to 10-32 Male PEEK

CHR4419 EACH
EUR 46.8

Control Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02 100 ug
EUR 170

V-Neck Polycotton Lab Coat - Female - 100cm/40in - Medium

SAF0078 EACH
EUR 61.2

Female reproductive system tumor tissue array

FE951 each
EUR 354
Description: Female reproductive system tumor tissue array, including pathology grade, TNM and clinical stage (AJCC 8.0), 95 cases/95 cores (core size 1.5mm), Recent Year Collection

Kinesis Adapter Kel-F M6 Female to 1/4-28 Male

CHR4337 EACH
EUR 39.6

Kinesis Adaptor Body - 1/4-28 Female x 1/4-28 Male

CHR4339 EACH
EUR 51.6

Kinesis Adaptor 1/4-28 Female to M6 Male

CHR4375 EACH
EUR 40.8

Kinesis Adapter PEEK 10.32 Male to M6 Female

CHR4301 EACH
EUR 22.8

Kinesis Adapter PEEK 10.32 Female to M6 Male

CHR4633 EACH
EUR 39.6

Kinesis Adapter 1/2-20 Female to 1/4-28 Male; PCTFE

CHR4361 EACH
EUR 39.6

Kinesis Adapter Body PEEK 10.32 Female to 1/4-28 Male

CHR4335 EACH
EUR 33.6

Adapter X2 M30x1.5 female to NPT 3/4" male

8890035 PK2
EUR 169.2

Adapter X2 M30x1.5 female to NPT 3/4" male

8890083 PK2
EUR 258

Human Hepatocytes-Single Donor, Female; Inducible

ABC-TC3648 1 vial Ask for price
Description: Gentaur's inducible hepatocytes (HP1 Heps-i, single donor, female) can be plated and used in long-term drug induction studies or metabolic studies where long-term incubations are required. Gentaur's cryopreserved primary human hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is tested for plating and provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available.

Kinesis Adaptor 5/16-24 Female To 1/4-28 Male; Kel-F

CHR4299 EACH
EUR 44.4

Kinesis Adapter 5/16-24 Female to 1/4-28 Male Kel-F

CHR4355 EACH
EUR 33.6

Kinesis Adapter Male luer to M6 Female ETFE

CHR4351 EACH
EUR 16.8

Astrocytes Mature (iPSC from Blood Cells; Female)

ASE-9322MF 1 vial (1 x 10^6)
EUR 546
Description: 12 month

Normal female dog multiple organs tissue array

DGF281 each
EUR 306
Description: Normal female dog multiple organs tissue array, 28 organs/ 28cores

Kinesis Adapter Male luer to 10-32 Female PEEK

CHR4309 EACH
EUR 21.6

Kinesis Adaptor PTFE 1/4-28 Female x 1/4-28 Male long

CHR4373 EACH
EUR 43.2

Neural Stem Cells (iPSC from Blood Cells; Female)

ASE-9234F 1 vial (1 x 10^6)
EUR 497.4
Description: 12 month

Dopaminergic Neurons (iPSC from Blood Cells; Female)

ASE-9323F 1 vial
EUR 448.8
Description: 12 month

Tubing 1mMetal insulated 2 fittings M16x1 female

8930221 KIT
EUR 340.8

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female

CHR4303 EACH
EUR 22.8

Kinesis Female Nut PEEK Black 1/4-28 - 1/8

CHR3357 EACH
EUR 12

Astrocytes Precursors (iPSC from Blood Cells; Female)

ASE-9322PF 1 vial (1 x 10^6)
EUR 448.8
Description: 12 month

Kinesis Female Nut PEEK Nat for 1/16in Tubing

CHR4141 EACH
EUR 13.2

Kinesis Adapter Female luer to Male 10-32 ETFE

CHR4287 EACH
EUR 8.4

Neurons Starter Kit (iPSC from Blood Cells; Female)

ASE-9321KF 1 Kit
EUR 473.1
Description: 12 month

Kinesis Adapter Male Luer to 1/4-28 Female PEEK

CHR4307 EACH
EUR 21.6

Kinesis Adapter Male luer to 1/4-28 Female ETFE

CHR4347 EACH
EUR 14.4

Kinesis Adapter Female Luer to Male 1/4-28; PP

CHR4241 EACH
EUR 8.4

Kinesis Adapter Female Luer to Male 1/4-28; ETFE

CHR4255 EACH
EUR 8.4

Kinesis Adaptor; Female Luer to 5/16-24 Male; ETFE

CHR4319 EACH
EUR 8.4

Kinesis Adapter PP; Barb 1/16 to Luer Lock Female

CHR4589 EACH
EUR 2.4

V-Neck Polycotton Lab Coat - Female - 92cm/36in - Small

SAF0074 EACH
EUR 62.4

Kinesis Adapter PEEK 10.32 Flat Male to 1/4-28 Female

CHR4341 EACH
EUR 39.6

V-Neck Polycotton Lab Coat - Female - 96cm/38in - Medium

SAF0076 EACH
EUR 60

Kinesis Adapter Female Luer to Male 1/4-28; Delrin

CHR4231 EACH
EUR 7.2

Multiplex ELISA Kit For Human Female Hormone - (8-plex)

MEK1022 1 kit
EUR 1696.8

Kinesis Adaptor 5/16-24 Male To 1/4-28 Female; Kel-F

CHR4417 EACH
EUR 39.6

Kinesis Adapter PEEK 10.32 Male to 1/4-28 Female; Extra Long

CHR4305 EACH
EUR 25.2

Kinesis Female Luer to Barb Adapter 1/16ID Tubing; Tefzel

CHR4605 EACH
EUR 7.2

Dopaminergic Neurons Starter Kit (iPSC from Blood Cells; Female)

ASE-9323KF 1 vial
EUR 546
Description: 12 month

Astrocytes Precursors Starter Kit (iPSC from Blood Cells; Female)

ASE-9322PKF 1 Kit
EUR 497.4
Description: 12 month

Kinesis Adapter Female Luer to Male 1/4-28; PP; Large Bore

CHR4243 EACH
EUR 8.4

Kinesis Adapter Assembly 1/8 NPT x 1/4-28 Female PEEK Red

CHR5065 EACH
EUR 25.2

Kinesis Adapter Assembly 1/4in NPT x 1/4-28 Female PEEK Red

CHR5053 EACH
EUR 30

Dimethyl ether 99% cylinder with 1/4" brass hose adapter (female)

D40060 100G
EUR 790.2

Monkey Capuchin (Cebus) control (non-immunized) serum, Male and Female

NMCB-1 0.5 ml
EUR 196.8

CELLSTACK,FILLING ACCESSORY,3/8 IN C-FLEX,FEMALE MPC,S,1/4

11902 1/pk
EUR 337.2
Description: Stackable Cell Culture; Closed Systems Cell Culture Chambers

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-5 5 slides
EUR 883

C-FLEX TUBING, 3'- 1/4" ID, WITH MALE LUER WITH CAP TO FEMALE LUER

91-700-00 1/pk
EUR 79.2
Description: Flexible Containers; Bioprocess Bags and Parts

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I

T6834700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I

T6834700-G02-5 5 slides
EUR 883

Elbow fittings x2 90° M16x1 female/male stainless steel side length 2x54mm

8970442 PK2
EUR 262.8

Option quick disconnect kit, includes 2 male/female attachments & silicone tubing

V0020-QD 1 PC
EUR 144.9

HeLa Cell Slide (Human (31 yrs, Female) cervix epithelioid carcinoma) (5 slides/pk)

HCLS-17001 1 pk
EUR 300

Monkey Marmoset (Callithrix jacchus) control (non-immunized) serum, Male and Female

NMMS-1 0.5 ml
EUR 270

A-431 Cell Slide (Human (85 yrs, Female) skin epidermoid carcinoma) (5 slides/pk)

HCLS-17002 1 pk
EUR 300

CELLSTACK,FILLING ACC,33MM CAP,MPC FEMALE QK CON,W/DUSTCVR,3/8"ID,S,IND,1/4

3329 1/pk
EUR 337.2
Description: Stackable Cell Culture; CellSTACK

Option quick disconnect kit, includes 2 male/female attachments and silicone tubing

BCM1550 1 pcs, 1 UNIT
EUR 244.87

Monkey African Green (Chlorocebus) control (non-immunized) serum, Male and Female Available

NMAG-1 1 ml
EUR 169.2

L1210 Cell Slide (DBA subline 212 Mouse (8 months, Female) lymphocytic leukemia) (5 slides/pk)

MCLS-17204 1 pk
EUR 300

Corning 33mm Filling Cap 1/4inch (6.4mm) ID Tubing a Male MPC Coupling and a Female MPC End Cap

3333 PK4
EUR 355.2

MCF7 Cell Slide (Human (69 yrs, Female) mammary gland, breast, epithelial adenocarcinoma) (5 slides/pk)

HCLS-17012 1 pk
EUR 300

Ecm2 (GFP-tagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2)

MG209923 10 µg Ask for price

K-562 Cell Slide (Human (53yrs, Female) bone marrow, chronic myelogenous leukemia (CML)) (5 slides/pk)

HCLS-17004 1 pk
EUR 300

Ecm2 (untagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2), (10ug)

MC220394 10 µg Ask for price

Corning 33mm Filling Cap with a Female MPC with a 1/4inch (6.4mm) ID Coupling and a Male MPC End Cap

3328 PK4
EUR 333.6

Ecm2 (Myc-DDK-tagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2)

MR209923 10 µg Ask for price

Rat Liver Cells

ABC-TC4151 1 vial Ask for price
Description: Rat primary liver cells (RPLIC) are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Rat primary liver cells are mixed population of mouse liver cells grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubate in Culture Complete Growth Medium.

C-FLEX TUBING, 2'-1/4" ID, WITH FEMALE MPC CONNECTOR WITH 1/4" BARB WITH PINCH CLAMP AND FILLING BELL

91-700-12 1/pk
EUR 231.6
Description: Flexible Containers; Bioprocess Bags and Parts

Corning 33mm Filling Cap 1/8inch ID Tubing and a Female Luer 1/8inch Hose Barb a Male Luer Lock Plug

3282 PK5
EUR 267.6

Rat liver fibroblasts

ABC-TC4153 1 vial Ask for price
Description: Rat liver fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Mouse liver fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

HL-60 Cell Slide (Human (36yrs, Female) peripheral blood promyeloblast, acute promyelocytic leukemia) (5 slides/pk)

HCLS-17009 1 pk
EUR 300

ECM2 (untagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 1

SC303009 10 µg Ask for price

Lenti ORF clone of Ecm2 (mGFP-tagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2)

MR209923L4 10 µg Ask for price

3`UTR clone of extracellular matrix protein 2 female organ and adipocyte specific (ECM2) for miRNA target validation

SC211695 10 µg Ask for price

Rat Liver Tissue Lysate

LYSATE0003 200ug
EUR 180
Description: This cell lysate is prepared from rat liver tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Liver Tissue Lysate

IRTLRTL100UG each
EUR 245
Description: Rat Liver Tissue Lysate

Rat Liver Kupffer Cells

ABC-HP030X 1 vial Ask for price
Description: Rat Liver Kupffer Cells are isolated from the liver of 4-6 week old Sprague-Dawley rat. Rat Liver Kupffer Cells are cryopreserved at passage 0 and delivered frozen. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

OORA00537-1U - Rat LIVER

OORA00537-1U 1Each
EUR 60

ECM2 (GFP-tagged) - Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 1

RG217806 10 µg Ask for price

ECM2 (GFP-tagged) - Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 2

RG230864 10 µg Ask for price

ECM2 (GFP-tagged) - Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 3

RG231351 10 µg Ask for price

OORA00537-1EA - Rat LIVER

OORA00537-1EA 1Each
EUR 59

Rat Liver Epithelial Cells

ABC-TC4152 1 vial Ask for price
Description: Rat Liver Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Liver Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Liver Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Liver Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Lenti ORF clone of Ecm2 (Myc-DDK-tagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2)

MR209923L3 10 µg Ask for price

cDNA - Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 729

ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 1

RC217806 10 µg Ask for price

ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 3

RC231351 10 µg Ask for price

ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 2

RC230864 10 µg Ask for price

Liver Liver Cirrhosis Lysate

XBL-10358 0.1 mg
EUR 796.2
Description: Human liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human liver tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Rat Liver PrimaCell: Hepatocytes

2-82595 1 Kit Ask for price

Rat Ferritin (Liver) Antibody

11901-05011 150 ug
EUR 260.4

Rat Liver Whole Cell Lysate

IRTLRTLW500UG each
EUR 327
Description: Rat Liver Whole Cell Lysate

Rat Liver X receptor alpha

E01A14432 96T
EUR 700
Description: ELISA

Rat Liver glycogen ELISA kit

E02H0052-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit

E02H0052-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit

E02H0052-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Liver glycogen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Liver glycogen ELISA kit

E01A13744 96T
EUR 700
Description: ELISA

Total RNA - Liver Cirrhosis: Liver

R1236149Lcs-50 50 ug
EUR 421.2

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from 4 different donors, 2 male and 2 female. 1 ug/ul concentration

R1234148-10-P04 10x10 µg
EUR 2199

Rat Liver Whole tissue lysate

RAL-1464 1 mg
EUR 628.8

Total Protein - Liver Cirrhosis: Liver

P1236149Lcs 1 mg
EUR 471

Lenti ORF clone of Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 1, mGFP tagged

RC217806L4 10 µg Ask for price

Liver Membrane Liver Cirrhosis Lysate

XBL-10671 0.1 mg
EUR 752.1
Description: Human liver tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human liver tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated liver tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated liver tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 385

Membrane Protein - Liver Cirrhosis: Liver

P3236149Lcs 0.1 mg
EUR 410

Lenti ORF particles, Ecm2 (GFP-tagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2), 200ul, >10^7 TU/mL

MR209923L4V 200 µl Ask for price

Lenti ORF particles, Ecm2 (Myc-DDK-tagged) - Mouse extracellular matrix protein 2, female organ and adipocyte specific (Ecm2), 200ul, >10^7 TU/mL

MR209923L3V 200 µl Ask for price

Liver cancer and liver tissue array

BC03116a each
EUR 306
Description: Liver cancer and liver tissue array, including pathology grade, TNM and clinical stage, 70 cases/70 cores, replacing BC03116

Liver tumor with liver tissue array

T032d each
EUR 48
Description: Liver tumor with liver tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T032c

HighQC™ Rat Liver Stem Cell

ABC-SC0013 1 vial Ask for price
Description: Rat Liver Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.

Liver cancer with liver tissue array

LV1021a each
EUR 204
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 102 cases/102 cores, replacing LV1021

Liver cancer with liver tissue array

LV243 each
EUR 66
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores

Liver cancer with liver tissue array

LV722 each
EUR 306
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing LV721

Liver cancer with liver tissue array

LV803b each
EUR 270
Description: Liver cancer with liver tissue array, including pathology grade, TNM and clinical stage, 54 cases/72 cores, replacing LV803a

Rat Liver X Receptor ELISA kit

E01A14389 96T
EUR 700
Description: ELISA

Lenti ORF clone of Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 1, Myc-DDK-tagged

RC217806L3 10 µg Ask for price

Lenti-ORF clone of ECM2 (mGFP-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 3

RC231351L4 10 µg Ask for price

Lenti-ORF clone of ECM2 (mGFP-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 2

RC230864L4 10 µg Ask for price

Rat Liver Sinusoidal Endothelial Cells

ABC-TC4154 1 vial Ask for price
Description: Rat Liver Sinusoidal Endothelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal Sprague-Dawley Rats. Rat Liver Sinusoidal Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Rat Liver Sinusoidal Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Lenti ORF particles, ECM2 (Myc-DDK tagged) - Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant

RC217806L3V 200 µl Ask for price

Lenti ORF particles, ECM2 (mGFP-tagged) - Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 1,

RC217806L4V 200 µl Ask for price

Human Liver Cirrhosis Liver Tissue Lysate

IHULRCIRTL100UG each
EUR 1413
Description: Human Liver Cirrhosis Liver Tissue Lysate

Paraffin Tissue Section - Liver Cirrhosis: Liver

T2236149Lcs 5 slides
EUR 238

Lenti ORF particles, ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 3

RC231351L3V 200 µl Ask for price

Lenti ORF particles, ECM2 (mGFP-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 3, 2

RC231351L4V 200 µl Ask for price

Lenti ORF particles, ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 2

RC230864L3V 200 µl Ask for price

Lenti ORF particles, ECM2 (mGFP-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 2, 2

RC230864L4V 200 µl Ask for price

OCPB00553-100UG - Liver Liver Cirrhosis Lysate

OCPB00553-100UG 0.1mg
EUR 739

Rat Collectin Liver 1 ELISA kit

E02C0405-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit

E02C0405-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit

E02C0405-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Collectin Liver 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collectin Liver 1 ELISA kit

E01A10983 96T
EUR 700
Description: ELISA

Rat Phosphofructokinase Liver ELISA Kit

IRTPFKLKT each
EUR 790
Description: Rat Phosphofructokinase Liver ELISA Kit

Liver cancer and normal liver tissue array

LV483a each
EUR 198
Description: Liver cancer and normal liver tissue array, including hepatocellular carcinoma,cholangiocellular carcinoma and normal tissues pathology grade, TNM and clinical stage (AJCC 7th edition), 48 cases/48 cores (core size 1.5mm), replacing LV483

Lenti-ORF clone of ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 3

RC231351L3 10 µg Ask for price

Lenti-ORF clone of ECM2 (Myc-DDK-tagged)-Human extracellular matrix protein 2, female organ and adipocyte specific (ECM2), transcript variant 2

RC230864L3 10 µg Ask for price

Total RNA - Rat Normal Tissue: Liver

R1434149-50 50 ug
EUR 130

N-Glucuronide and sequential TZD ring-opened metabolites have been additionally present in liver microsomes within the presence of UDP-glucuronic acid. The O-demethyl-O-sulfate (M11), which is the main in vivo metabolite in rats and monkeys, was detected in all species of hepatocytes. As well as, a TZD ring-opened S-cysteine conjugate (M15) was detected in human hepatocytes. From these outcomes, the in vivo metabolic pathways in people have been predicted to be the 4 oxidation and one N-glucuronidation pathways. The 4 oxidative metabolites have been fashioned by a number of human P450 enzymes, and N-glucuronide was fashioned by UGT1A3 and UGT2B7.

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