Simian-human immunodeficiency virus (SHIV) an infection supplies a related animal mannequin to review HIV-1 neutralization breadth. With beforehand recognized SHIVSF162P3N contaminated rhesus macaques that did or didn’t develop neutralization breadth, we characterised the transmitted/founder viruses and preliminary autologous/homologous neutralizing antibodies in these animals.
The plasma viral load and blood CD4 rely didn’t distinguish macaques with and with out breadth, and just one examined homologous envelope clone revealed a development for macaques with breadth to favor an early homologous response.
In two macaques with breadth, GB40 and FF69, contaminated with uncloned SHIVSF162P3N, a number of viral variants have been transmitted, and the transmitted variants weren’t equal in neutralization sensitivity. The targets of preliminary autologous neutralizing antibodies, arising between 10 and 20 weeks put up an infection, have been mapped to N462 glycan and G460a in gp120 V5 in GB40 and FF69, respectively.
Though it’s unclear whether or not these targets are associated to later neutralization breadth growth, the G460a goal however not N462 glycan appeared extra widespread in macaques with breadth than these with out. Longitudinal plasmas revealed 2⁻three sequential waves of neutralizing antibodies in macaques with breadth, implicating that three sequential envelope variants, if no more, could also be required for the broadening of HIV-1 neutralizing antibodies.
Excessive-Decision Construction Evaluation of Antibody V5 and U4 Conformational Epitopes on Human Papillomavirus 16.
Cancers attributable to human papillomavirus (HPV) place an enormous burden on the well being of each women and men. The present business vaccines are genotype particular and supply little therapeutic profit to sufferers with current HPV infections.
Figuring out the conformational epitopes on the virus capsid helps the event of improved recombinant vaccines to maximise long-term safety towards a number of sorts of HPV. Fragments of antibody (Fab) digested from the neutralizing monoclonal antibodies H16.V5 (V5) and H16.U4 (U4) have been certain to HPV16 capsids and the constructions of the 2 virus-Fab complexes have been solved to close atomic decision utilizing cryo-electron microscopy.
The constructions reveal virus conformational adjustments, the Fab-binding mode to the capsid, the residues comprising the epitope and point out a possible interplay of U4 with the minor structural protein, L2. Competitors enzyme-linked immunosorbent assay (ELISA) confirmed V5 outcompetes U4 when added sequentially, demonstrating a steric interference though the footprints don’t overlap.
Mixed with our beforehand reported immunological and structural outcomes, we suggest that the virus could provoke host entry by way of an interplay between the icosahedral five-fold vertex of the capsid and receptors on the host cell. The extremely detailed epitopes recognized for the 2 antibodies present a framework for persevering with biochemical, genetic and biophysical research.
A 2-4-Amino Acid Deletion within the V5 Area of HIV-1 Env gp120 Confers Viral Resistance to the Broadly Neutralizing Human Monoclonal Antibody, VRC01.
The envelope glycoprotein (Env) gp120 of human immunodeficiency virus sort 1 (HIV-1) performs a essential function in viral entry into host cells. The broadly neutralizing human monoclonal antibody VRC01, which acknowledges the CD4 binding website on gp120, neutralizes greater than 90% of HIV-1 isolates.
Nevertheless, among the CRF01_AE viruses prevalent in Southeast Asia are proof against VRC01-mediated neutralization. We beforehand reported that three amino acid residues at positions 185, 186, and 197 of gp120 performed an necessary function within the VRC01 resistance of CRF01_AE Env (AE-Env) clones remoted from HIV-infected Thai people.
Nevertheless, the VRC01 susceptibility of AE-Env clones was not absolutely defined by mutations at these three residues. Within the current examine, we examined different components concerned within the acquisition of viral VRC01 resistance.
Neutralization assessments utilizing lentiviral vectors expressing a collection of mutant AE-Env clones revealed that the deletion of 2-Four amino acid residues on the loop construction within the V5 area of gp120 conferred VRC01 resistance to a number of AE-Env clones. Our outcomes present novel insights into the mechanisms underlying viral VRC01 resistance.
A cryo-electron microscopy examine identifies the whole H16.V5 epitope and divulges world conformational adjustments initiated by binding of the neutralizing antibody fragment.
Human papillomavirus 16 (HPV16) is a worldwide well being risk and an etiologic agent of cervical most cancers. To grasp the antigenic properties of HPV16, we pursued a structural examine to elucidate HPV capsids and antibody interactions.
The cryo-electron microscopy (cryo-EM) constructions of a mature HPV16 particle and an altered capsid particle have been solved individually and as complexes with fragment of antibody (Fab) from the neutralizing antibody H16.V5. Fitted crystal constructions offered a pseudoatomic mannequin of the virus-Fab advanced, which recognized a exact footprint of H16.V5, together with beforehand unrecognized residues.
The altered-capsid-Fab advanced map confirmed that binding of the Fab induced vital conformational adjustments that weren’t seen within the altered-capsid construction alone. These adjustments included extra ordered floor loops, consolidated so-called “invading-arm” constructions, and tighter intercapsomeric connections on the capsid flooring. The H16.
V5 Fab preferentially certain hexavalent capsomers probably with a stabilizing impact that straight correlated with the variety of certain Fabs. Extra cryo-EM reconstructions of the virus-Fab advanced for various incubation instances and structural evaluation present a mannequin for a hyperstabilization of the capsomer by H16.V5 Fab and confirmed that the Fab distinguishes refined variations between antigenic websites.
A single residue throughout the V5 area of HIV-1 envelope facilitates viral escape from the broadly neutralizing monoclonal antibody VRC01.
VRC01, a broadly neutralizing monoclonal antibody, is able to neutralizing a various array of HIV-1 isolates by mimicking CD4 binding with the envelope glycoprotein gp120. Nonetheless, resistant strains have been recognized. Right here, we examined two genetically associated and two unrelated envelope clones, derived from CRF08_BC-infected sufferers, with distinct VRC01 neutralization profiles.
A complete of 22 chimeric envelope clones was generated by interchanging the loop D and/or V5 areas between the unique envelopes or by single alanine substitutions inside every area. Evaluation of pseudoviruses constructed from these mutant envelopes confirmed that interchanging the V5 area between the genetically associated or unrelated clones fully swapped their VRC01 sensitivity profiles.
Mutagenesis evaluation revealed that the asparagine residue at place 460 (Asn-460), a possible N-linked glycosylation website within the V5 area, is a key issue for noticed resistance in these strains, which is additional supported by our structural modeling. Furthermore, adjustments in resistance have been discovered to positively correlate with deviations in VRC01 binding affinity.
 v5 Tag Antibody |
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abx018238-100ug |
Abbexa |
100 ug |
EUR 460.8 |
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 V5 Tag Antibody |
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abx034630-400ul |
Abbexa |
400 ul |
EUR 627.6 |
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V5 Tag Antibody |
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abx034630-80l |
Abbexa |
80 µl |
EUR 343.2 |
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|
 V5 tag Antibody |
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abx019189-100ug |
Abbexa |
100 ug |
EUR 385.2 |
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|
 V5 tag antibody |
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70R-12231 |
Fitzgerald |
100 ug |
EUR 523.2 |
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Description: Rabbit polyclonal V5 tag antibody |
 V5 Tag antibody |
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70R-32858 |
Fitzgerald |
100 ug |
EUR 301.2 |
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Description: Goat polyclonal V5 Tag antibody |
V5 Tag antibody |
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70R-36761 |
Fitzgerald |
100 ug |
EUR 418.8 |
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Description: Rabbit Polyclonal V5 Tag antibody |
V5 tag Antibody |
|
48731-100ul |
SAB |
100ul |
EUR 399.6 |
V5 tag Antibody |
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48731-50ul |
SAB |
50ul |
EUR 286.8 |
V5 Tag Antibody |
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F52064-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 140.25 |
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Description: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells. |
V5 Tag Antibody |
|
F52064-0.4ML |
NSJ Bioreagents |
0.4 ml |
EUR 322.15 |
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Description: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells. |
V5 Tag Antibody |
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RQ4680 |
NSJ Bioreagents |
100ul |
EUR 356.15 |
|
Description: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). |
 Anti-V5 antibody |
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STJ140012 |
St John's Laboratory |
300 µg |
EUR 324 |
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Description: Goat polyclonal antibody to V5 epitope. V5 peptide corresponds to amino acid residues GKPIPNPLLGLDST (95-108 aa) of the P and V proteins of the Paramyxovirus of simian virus SV5. |
 V5-tag Antibody |
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20-abx134695 |
Abbexa |
-
EUR 427.20
-
EUR 678.00
-
EUR 360.00
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V5-tag Antibody |
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20-abx134696 |
Abbexa |
-
EUR 427.20
-
EUR 678.00
-
EUR 360.00
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|
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V5-tag Antibody |
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20-abx134697 |
Abbexa |
-
EUR 427.20
-
EUR 644.40
-
EUR 260.40
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V5-tag Antibody |
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abx239354-100ug |
Abbexa |
100 ug |
EUR 661.2 |
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|
V5-tag Antibody |
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abx239355-100ug |
Abbexa |
100 ug |
EUR 661.2 |
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|
 V5-Tag Antibody |
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20-abx242880 |
Abbexa |
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V5-Tag Antibody |
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20-abx242894 |
Abbexa |
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V5-Tag Antibody |
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3985-100 |
Biovision |
each |
EUR 405.6 |
V5-Tag Antibody |
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3985-30T |
Biovision |
each |
EUR 175.2 |
V5-Tag Antibody |
|
48047-100ul |
SAB |
100ul |
EUR 399.6 |
V5-Tag Antibody |
|
48047-50ul |
SAB |
50ul |
EUR 286.8 |
 V5-Tag Mouse Monoclonal Antibody, Clone V5.E10 |
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Ab1008-100 |
GenDepot |
100ug |
EUR 457.2 |
V5-Tag Mouse Monoclonal Antibody, Clone V5.E10 |
|
Ab1008-101 |
GenDepot |
1mg |
EUR 2733.6 |
) V5 Tag Antibody (AP) |
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abx412990-01mg |
Abbexa |
0.1 mg |
EUR 727.2 |
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|
 anti- V5-tag antibody |
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FNab09354 |
FN Test |
100µg |
EUR 702 |
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|
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Description: Antibody raised against V5-tag |
anti- V5-tag antibody |
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FNab09355 |
FN Test |
100µg |
EUR 702 |
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|
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Description: Antibody raised against V5-tag |
) V5 Tag Antibody (FITC) |
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abx412992-01mg |
Abbexa |
0.1 mg |
EUR 710.4 |
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V5 Tag Antibody (FITC) |
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abx414671-01mg |
Abbexa |
0.1 mg |
EUR 710.4 |
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V5 Tag Antibody (FITC) |
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abx414674-01mg |
Abbexa |
0.1 mg |
EUR 710.4 |
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 Anti-V5-Tag antibody |
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STJ96907 |
St John's Laboratory |
200 µl |
EUR 236.4 |
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Description: Mouse monoclonal to V5-Tag. |
Anti-V5-Tag antibody |
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STJ96924 |
St John's Laboratory |
200 µl |
EUR 236.4 |
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Description: Mouse monoclonal to V5-Tag. |
Anti-V5-Tag antibody |
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STJ97045 |
St John's Laboratory |
200 µl |
EUR 236.4 |
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Description: Mouse monoclonal to V5-Tag. |
 V5-Tag HRP Antibody |
|
48053-100ul |
SAB |
100ul |
EUR 399.6 |
V5-Tag HRP Antibody |
|
48053-50ul |
SAB |
50ul |
EUR 286.8 |
Total, our examine signifies that Asn-460 within the V5 area is a essential determinant of sensitivity to VRC01 particularly in these viral strains. The lengthy aspect chain of Asn-460, and potential glycosylation, could create steric hindrance that lowers binding affinity, thereby growing resistance to VRC01 neutralization.
