Microarray patches allow the event of skin-targeted vaccines in opposition to COVID-19
The COVID-19 pandemic is a severe risk to international well being and the worldwide economic system. The continuing race to develop a secure and efficacious vaccine to stop an infection by SARS-CoV-2, the causative agent for COVID-19, highlights the significance of vaccination to fight infectious pathogens. The extremely accessible cutaneous microenvironment is a perfect goal for vaccination because the pores and skin harbors a excessive density of antigen-presenting cells and immune accent cells with broad innate immune capabilities. Microarray patches (MAPs) are a gorgeous intracutaneous biocargo supply system that permits secure, reproducible, and managed administration of vaccine elements (antigens, with or with out adjuvants) to outlined pores and skin microenvironments.
This assessment describes the construction of the SARS-CoV-2 virus and related antigenic targets for vaccination, summarizes key ideas of pores and skin immunobiology within the context of prophylactic immunization, and presents an summary of MAP-mediated cutaneous vaccine supply. Concluding remarks on MAP-based pores and skin immunization are supplied to contribute to the rational improvement of secure and efficient MAP-delivered vaccines in opposition to rising infectious illnesses, together with COVID-19.
Substitutional panorama of a break up fluorescent protein fragment utilizing high-density peptide microarrays
Break up fluorescent proteins have huge applicability as biosensors for protein-protein interactions, genetically encoded tags for protein detection and localization, in addition to fusion companions in super-resolution microscopy. We’ve got right here established and validated a novel platform for useful evaluation of leave-one-out break up fluorescent proteins (LOO-FPs) in excessive throughput and with speedy turnover. We’ve got screened greater than 12,000 variants of the beta-strand break up fragment utilizing high-density peptide microarrays for binding and useful complementation in Inexperienced Fluorescent Protein. We studied the impact of peptide size and the impact of various linkers to the strong help. We additional mapped the impact of all potential amino acid substitutions on every place in addition to within the context of some single and double amino acid substitutions.
As all peptides have been examined in 12 duplicates, the evaluation rests on a agency statistical foundation permitting for affirmation of the robustness and precision of the strategy. Based mostly on experiments in resolution, we conclude that beneath the given circumstances, the sign depth on the peptide microarray faithfully displays the binding affinity between the break up fragments. With this, we’re in a position to establish a peptide with 9-fold greater affinity than the beginning peptide.
aetox
Screening and identification of potential biomarkers for obstructive sleep apnea by way of microarray evaluation
Obstructive sleep apnea (OSA) is a standard continual illness and will increase the chance of heart problems, metabolic and neuropsychiatric problems, leading to a substantial socioeconomic burden. This examine aimed to establish potential key genes affect the mechanisms and penalties of OSA.Gene expression profiles associated to OSA have been obtained from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) in subcutaneous adipose tissues from OSA in contrast with regular tissues have been screened utilizing R software program, adopted by gene ontology (GO) and pathway enrichment analyses. Subsequently, a protein-protein interplay (PPI) community for these DEGs was constructed by STRING, and key hub genes have been extracted from the community with plugins in Cytoscape.
The hub genes have been additional validated in one other GEO dataset and assessed by receiver working attribute (ROC) evaluation and Pearson correlation evaluation.There have been 373 DEGs in OSA samples in relative to regular controls, which have been primarily related to olfactory receptor exercise and olfactory transduction. Upon analyses of the PPI community, GDNF, SLC2A2, PRL, and SST have been recognized as key hub genes. Decreased expression of the hub genes was affiliation with OSA prevalence, and exhibited good efficiency in distinguishing OSA from regular samples primarily based on ROC evaluation. Moreover, the Pearson technique revealed a robust correlation between hub genes, which signifies that they could act in synergy, contributing to OSA and associated problems.This bioinformatics analysis recognized Four hub genes, together with GDNF, SLC2A2, PRL, and SST which can be new potential biomarkers for OSA and associated problems.
Identification of novel biomarkers and candidate small-molecule medicine in cutaneous melanoma by complete gene microarrays evaluation
Background: Melanoma is a pernicious pores and skin most cancers with excessive aggressiveness. This examine aimed to establish potential novel biomarkers related to the prognosis and pathogenesis of cutaneous melanoma and to discover new focused medicine for melanoma.
Strategies: Two Gene Expression Omnibus (GEO) microarray datasets, GSE3189 and GSE7553 have been mixed to investigate the differentially expressed genes (DEGs). To raised perceive the DEGs within the melanoma pathogenesis, we carried out gene enrichment analyses and established a protein-protein interplay community (PPI). The survival analyses for key genes have been carried out primarily based on the GEPIA platform. Lastly, we mined the CMap database to discover potential small-molecule medicine to focus on the obtained DEGs.
Outcomes: Briefly, we recognized 500 DEGs between cutaneous melanoma samples and regular samples. The PPI community was established with 349 nodes and 1251 edges. Signaling pathway evaluation confirmed that these genes play a significant position in ECM-receptor interactions, the PPAR signaling pathway and pathways in most cancers.
Eight DEGs with a comparatively excessive diploma of connectivity (CDC45, CENPF, DTL, FANCI, GINS2, HJURP, TPX2 and TRIP13) have been chosen as hub-genes that remarkably correlated to a poor survival fee. Based mostly on 500 DEGs, 20 small-molecule medicine that doubtlessly goal genes with irregular expression in cutaneous melanoma have been obtained from the CMap database. Amongst these compounds, we discovered that menadione has the best therapeutic worth for melanoma.
Conclusions: In conclusion, we recognized the eight candidate biomarkers and potential key signaling pathways in cutaneous melanoma by complete microarray analyses. The recognized candidate medicine have supplied a number of directive significances for the synthesis drugs for melanoma.
Universal control tissue microarray |
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| CTRL141 | TissueArray | each | EUR 54 |
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Description: Universal control tissue microarray, 7 types of Immune System normal tissues, 7 cases/14 cores |
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Stomach adenocarcinoma tissue microarray |
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| ST991b | TissueArray | each | EUR 270 |
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Description: Stomach adenocarcinoma tissue microarray, including TNM, clinical stage and pathology grade, 33 cases/ 99cores, replacing ST991a |
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Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue I |
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| T6234431 | Biochain | 5 slides | EUR 590.4 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue II |
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| T6234432 | Biochain | 5 slides | EUR 590.4 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue III |
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| T6234433 | Biochain | 5 slides | EUR 590.4 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue III |
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| T6534423 | Biochain | 5 slides | EUR 588 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue III |
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| T6534423-Cy | Biochain | 5 slides | EUR 588 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue I |
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| T6534448 | Biochain | 5 slides | EUR 588 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue I |
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| T6534448-Cy | Biochain | 5 slides | EUR 588 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue I, 7 different tissues |
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| T6236444Alz | Biochain | 5 slides | EUR 1249.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue II, 7 different tissues |
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| T6236445Alz | Biochain | 5 slides | EUR 1249.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues |
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| T6236446Alz | Biochain | 5 slides | EUR 1249.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue IV, 7 different tissues |
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| T6236564Alz | Biochain | 5 slides | EUR 1249.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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High density tissue microarray of Hodgkin's Disease |
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| LM208 | TissueArray | each | EUR 546 |
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Description: High density tissue microarray of Hodgkin's Disease, Non-Hodgkin's lymphoma and normal lymph node tissues, 69 cases/208 cores |
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Stomach carcinoma with matched stomach tissue microarray |
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| ST2084b | TissueArray | each | EUR 546 |
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Description: Stomach carcinoma with matched stomach tissue microarray, containing 94 cases of adenocarcinoma, 1 each of signet ring cell carcinoma and squamous cell carcinoma, duplicated cores per case |
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Paraffin Tissue Section Panel - Mouse Whole Brain Segmentation Panel |
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| T8334035 | Biochain | 5 slides | EUR 667.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Syphilis Mixed Titer Panel (15 X 1mL) |
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| KZMC002 | Zeptometrix | 15 X 1mL | EUR 791.33 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
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Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue III |
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| T8334423 | Biochain | 5 slides | EUR 313.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue I |
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| T8334447 | Biochain | 5 slides | EUR 313.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue II |
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| T8334608 | Biochain | 5 slides | EUR 313.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Adrenal |
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| T1236004Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Brain |
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| T1236035Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Colon |
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| T1236090Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Esophagus |
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| T1236106Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Eye |
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| T1236108Dia | Biochain | 5 slides | EUR 753.6 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Heart |
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| T1236122Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Kidney |
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| T1236142Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Liver |
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| T1236149Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Lung |
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| T1236152Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Pancreas |
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| T1236188Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Skin |
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| T1236218Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Spleen |
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| T1236246Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Diabetic Tissue: Stomach |
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| T1236248Dia | Biochain | 5 slides | EUR 558 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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