Golgi-Cox impregnation1, 2 has been one of the most effective techniques for studying the normal and abnormal morphology of neurons and glia. Using the Golgi technique, subtle morphological alterations have been discovered in neuronal dendrites and dendritic spines in the brain of animals treated with drugs, as well as in the post-mortem brain of patients with neurological diseases.3, 4 However, the lack of reliability and the slow process of Golgi staining have been major obstacles to the widespread application of this technique.
The FD Rapid GolgiStain kit is designed according to the principle of the methods described by Ramón-Moliner2, Glaser and Van der Loos5. Not only has this kit drastically improved and simplified the Golgi-Cox technique, it has also proven to be extremely reliable and sensitive in demonstrating the morphological details of neurons and glia, especially dendritic spines. The FD Rapid GolgiStain Kit has been extensively tested and used extensively in the brains of various species of animals, as well as in post mortem human brain specimens.
Store at room temperature
Solution A 250 ml
Solution B 250 ml
Solution C 250 ml x 2
Solution D 250 ml
Solution E 250 ml
Glass Specimen Retriever 2
Natural hair paintbrush 2
Dropping bottle 1
User Manual 1
Materials required but not included:
- Double distilled or deionized water.
- Plastic or glass tubes or vials.
- Histological supplies and equipment, including gelatin-coated microscope slides, coverslips, staining jars, ethanol, xylene or xylene substitutes, resinous mounting medium (e.g. Permount®), and a light microscope.