Comparison of the sensitivity of Western blotting between PVDF and NC membranes

Comparison of the sensitivity of Western blotting between PVDF and NC membranes post thumbnail image
Western blotting (WB) is likely one of the most generally used methods to determine proteins in addition to submit translational modifications of proteins. The number of electroblotted membrane is likely one of the key elements affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The commonest used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. Which membrane of those two is extra appropriate for WB has not been reported up to now.
Right here, by incubating proteins which had been transferred to PVDF or NC membranes with a sequence of antibodies and several types of lectins, we investigated the connection between the binding means of those two membranes to proteins or glycoproteins and the molecular weight of the goal protein.
The antibody re-probed means of the 2 membranes was additionally explored. Furthermore, we verified the above outcomes by immediately incubating proteins having totally different molecular weights onto PVDF or NC membranes. Certain proteins had been stained with direct blue-71, and the staining depth was quantitated by scanning and densitometry.

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