Unconventional Cadherin Localization in Honey Bee Gonads Revealed Through Domain-Specific Apis mellifera E- and N-Cadherin Antibodies Indicates Alternative Functions.

Unconventional Cadherin Localization in Honey Bee Gonads Revealed Through Domain-Specific Apis mellifera E- and N-Cadherin Antibodies Indicates Alternative Functions. post thumbnail image
As key elements in intercellular adhesion processes, cadherins play vital roles in a plethora of developmental processes, together with gametogenesis. In a earlier examine on cadherin localization within the gonads of honey bees, carried out with heterologous pan-cadherin antibodies, we detected these proteins as (i) related to cell membranes, (ii) as homogeneously distributed all through the cytoplasm, and (iii) as nuclear foci in each somatic and germline cells, elevating the potential of various features.
To additional examine such uncommon intracellular cadherin localization we produced particular antibodies towards the N- and C-terminal domains of honey bee N- and E-cadherin. A 160 kDa protein was acknowledged by the E-cadherin antibodies in addition to considered one of roughly 300 kDa from these raised towards N-cadherin.
In gonad preparations, each proteins have been detected as dispersed all through the cytoplasm and as nuclear foci in each germline and somatic cells of queen and employee ovarioles, in addition to within the testioles of drones. This leads us to deduce that cadherins could certainly be concerned in sure signaling pathways and/or transcriptional regulation throughout gametogenesis.
In late oogenesis phases, immunolabeling for each proteins was noticed on the cell cortex, in conformity with a job in cell adhesion. In testioles, E-cadherin was seen in co-localization with fusomes, indicating a attainable function in cyst group. Taken collectively, the distribution of N- and E-cadherins in honey bee gonads is suggestive of different roles for cadherins in gametogenesis of each sexes.

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