Asociación Española de Toxicología (AETOX) ISSN: 1697-0748

S9 fraction rat hepatocyte microsomes, slices, p450, beagle

In vitro pharmacokinetic profile of a benzopyridooxathiazepine derivative using rat microsomes and hepatocytes: identification of phases I and II metabolites.

Within the current examine, the in vitro metabolic habits of a benzopyridooxathiazepine (BZN), a potent tubulin polymerization inhibitor, was investigated by liquid chromatography-UV detection (LC-UV). First, easy and quick LC-UV strategies have been optimized and validated to judge the pharmacokinetic profile of BZN utilizing rat liver microsomes or hepatocytes main cultures suspensions. Regardless of the medium investigated, baseline decision...