Asociación Española de Toxicología (AETOX) ISSN: 1697-0748

S9 fraction rat hepatocyte microsomes, slices, p450, beagle

Biosynthesis of cytochrome P-450 on membrane-bound ribosomes and its subsequent incorporation into rough and smooth microsomes in rat hepatocytes.

Intracellular websites of synthesis of cytochrome P-450 and the next incorporation of it into membrane buildings of the endoplasmic reticulum (ER) in rat hepatocytes have been studied utilizing an antibody monospecific for phenobarbital-inducible cytochrome P-450. The cytochrome is synthesized primarily on the "tightly certain" sort of membrane-bound ribosomes whose launch from the membrane requires therapy with puromycin in a excessive salt...