Asociación Española de Toxicología (AETOX) ISSN: 1697-0748

S9 fraction rat hepatocyte microsomes, slices, p450, beagle

Anti-anti-idiotypic (Ab3) antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from antibodies raised directly against the antigen.

Polyclonal rabbit anti-idiotypic (Ab2) antibodies raised in opposition to the antiprogesterone mAb DB3 (Ab1) had been used to induce an Ab3 antiprogesterone response in BALB/c mice. Whereas the affinity of Ab3 sera for progesterone was 10-50-times decrease than that of DB3, their steroid-binding specificity confirmed appreciable similarity to DB3. Two immunoglobulin M (IgM) Ab3 monoclonal antibodies (mAbs), 1A4 and 3B11, had been obtained, each...