Asociación Española de Toxicología (AETOX) ISSN: 1697-0748

S9 fraction rat hepatocyte microsomes, slices, p450, beagle

Pharmacokinetic interaction studies of tanshinones with tolbutamide, a model CYP2C11 probe substrate, using liver microsomes, primary hepatocytes and in vivo in the rat.

The results of Danshen and its lively parts (tanshinone I, tanshinone IIA, dihydrotanshinone and cryptotanshinone) on tolbutamide 4-hydroxylation was investigated within the rat. Danshen (0.125-2mg/ml) decreased 4-hydroxy-tolbutamide formation in vitro and in vivo. Enzyme kinetics research confirmed that inhibition of tolbutamide 4-hydroxylase exercise was aggressive and concentration-dependent. The Ok(i) values of the tanshinones have been:...